BBa_J36802
1
KaiB
KaiB coding region
2006-10-30T12:00:00Z
2015-08-31T04:08:47Z
synthetic; GeneArt AG
KaiB coding region with an extra stop codon at the end. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pPCR-Script (ampR). The biobricks primers can be cut out along with the KaiB coding sequence with KpnI and SacI. The length of the entire plasmid is 3220bp.
false
false
_65_
0
1189
65
Not in stock
false
-none-
false
Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_J36832
1
Lac+RBS+Ka
Lac+RBS+KaiB
2006-10-25T11:00:00Z
2015-08-31T04:08:47Z
Synthetic; GeneArt AG
KaiB coding region with an extra stop codon at the end. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG.
false
false
_65_
0
1189
65
Not in stock
true
Problems extracting KaiB from genome.
false
Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos
component1909002
1
BBa_R0010
component1909010
1
BBa_B0034
component1909011
1
BBa_J36802
annotation1909010
1
BBa_B0034
range1909010
1
209
220
annotation1909002
1
BBa_R0010
range1909002
1
1
200
annotation1909011
1
BBa_J36802
range1909011
1
227
538
BBa_J36832_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacgacttctaataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J36802_sequence
1
atgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacgacttctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z