LOCUS       BBa_J36849              1211 bp    DNA     linear       14-May-2024
DEFINITION  Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin 
            single-chain dimeric + His6 tag
ACCESSION   BBa_J36849
VERSION     BBa_J36849.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..313
                     /label=BBa_J36835
     CDS             322..384
                     /label=BBa_J36836
     CDS             393..1211
                     /label=BBa_J36843
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aagctactaa
      241 actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct ccagcaacgc
      301 taaaatcgat cagtactaga gaacccgtat gttggctttg aaatgggtta cgactggtta
      361 ggtcgtatgc cgtacaaagg cagctactag aggaggccaa cgccaagaag tccacgctgg
      421 tcggccacga caccttcacc aaggtgaagc cgtccgccgc ctccggtggt ggatctgcgg
      481 aggtcggcat caccggcacc tggtacaacc agctcggctc gaccttcatc gtgaccgcgg
      541 gcgccgacgg cgccctgacc ggaacctacg agtcggccgt cggcaacgcc gagagccgct
      601 acgtcctgac cggtcgttac gacagcgccc cggccaccga cggcagcggc accgccctcg
      661 gttggacggt ggcctggaag aataactacc gcaacgccca ctccgcgacc acgtggagcg
      721 gccagtacgt cggcggcgcc gaggcgagga tcaacaccca gtggctgctg acctccggca
      781 ccggctccgg aaccgccctc ggttggacgg tggcctggaa gaataactac cgcaacgccc
      841 actccgcgac cacgtggagc ggccagtacg tcggcggcgc cgaggcgagg atcaacaccc
      901 agtggctgct gacctccggc accaccgagg ccaacgcctg gaagtccacg ctggtcggcc
      961 acgacacctt caccaaggtg aagccgtccg ccgcctccgg tggtggatct gcggaggtcg
     1021 gcatcaccgg cacctggtac aaccagctcg gctcgacctt catcgtgacc gcgggcgccg
     1081 acggcgccct gaccggaacc tacgagtcgg ccgtcggcaa cgccgagagc cgctacgtcc
     1141 tgaccggtcg ttacgacagc gccccggcca ccgacggcgc ggccgcactc gagcaccacc
     1201 accaccactg a
//