LOCUS       BBa_J36850              1076 bp    DNA     linear       26-Apr-2024
DEFINITION  Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin wild-type 
            + His6 tag
ACCESSION   BBa_J36850
VERSION     BBa_J36850.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..313
                     /label=BBa_J36835
     CDS             322..663
                     /label=BBa_J36837
     CDS             672..1076
                     /label=BBa_J36841
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aagctactaa
      241 actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct ccagcaacgc
      301 taaaatcgat cagtactaga gaacccgtat gttggctttg aaatgggtta cgactggtta
      361 ggtcgtatgc cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa
      421 ctgaccgcta aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt
      481 ggcatggtat ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc
      541 gtttctccgg tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt
      601 ctggaatacc agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac
      661 aactactaga ggctgaagct ggtatcaccg gcacctggta caaccagctg ggatccacct
      721 tcatcgttac cgctggtgct gacggtgctc tgaccggtac ctacgaatcc gctgttggta
      781 acgctgaaag ccgctacgtt ctgaccggtc gttacgactc cgctccggct accgacggtt
      841 ccggaaccgc tctgggttgg accgttgctt ggaaaaacaa ctaccgtaac gctcactccg
      901 ctaccacctg gtctggccag tacgttggtg gtgctgaagc tcgtatcaac acccagtggt
      961 tgttgacctc cggcaccacc gaagccaacg cgtggaaatc caccctggtt ggtcacgaca
     1021 ccttcaccaa agttaaaccg tccgctgctt cccatcacca tcaccaccat taataa
//