LOCUS BBa_J36851 1490 bp DNA linear 22-Nov-2024 DEFINITION Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag ACCESSION BBa_J36851 VERSION BBa_J36851.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..313 /label=BBa_J36835 CDS 322..663 /label=BBa_J36837 CDS 672..1490 /label=BBa_J36843 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aagctactaa 241 actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct ccagcaacgc 301 taaaatcgat cagtactaga gaacccgtat gttggctttg aaatgggtta cgactggtta 361 ggtcgtatgc cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa 421 ctgaccgcta aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt 481 ggcatggtat ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc 541 gtttctccgg tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt 601 ctggaatacc agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac 661 aactactaga ggaggccaac gccaagaagt ccacgctggt cggccacgac accttcacca 721 aggtgaagcc gtccgccgcc tccggtggtg gatctgcgga ggtcggcatc accggcacct 781 ggtacaacca gctcggctcg accttcatcg tgaccgcggg cgccgacggc gccctgaccg 841 gaacctacga gtcggccgtc ggcaacgccg agagccgcta cgtcctgacc ggtcgttacg 901 acagcgcccc ggccaccgac ggcagcggca ccgccctcgg ttggacggtg gcctggaaga 961 ataactaccg caacgcccac tccgcgacca cgtggagcgg ccagtacgtc ggcggcgccg 1021 aggcgaggat caacacccag tggctgctga cctccggcac cggctccgga accgccctcg 1081 gttggacggt ggcctggaag aataactacc gcaacgccca ctccgcgacc acgtggagcg 1141 gccagtacgt cggcggcgcc gaggcgagga tcaacaccca gtggctgctg acctccggca 1201 ccaccgaggc caacgcctgg aagtccacgc tggtcggcca cgacaccttc accaaggtga 1261 agccgtccgc cgcctccggt ggtggatctg cggaggtcgg catcaccggc acctggtaca 1321 accagctcgg ctcgaccttc atcgtgaccg cgggcgccga cggcgccctg accggaacct 1381 acgagtcggc cgtcggcaac gccgagagcc gctacgtcct gaccggtcgt tacgacagcg 1441 ccccggccac cgacggcgcg gccgcactcg agcaccacca ccaccactga //