LOCUS       BBa_J36851              1490 bp    DNA     linear       10-May-2024
DEFINITION  Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin 
            single-chain dimeric + His6 tag
ACCESSION   BBa_J36851
VERSION     BBa_J36851.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..313
                     /label=BBa_J36835
     CDS             322..663
                     /label=BBa_J36837
     CDS             672..1490
                     /label=BBa_J36843
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aagctactaa
      241 actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct ccagcaacgc
      301 taaaatcgat cagtactaga gaacccgtat gttggctttg aaatgggtta cgactggtta
      361 ggtcgtatgc cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa
      421 ctgaccgcta aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt
      481 ggcatggtat ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc
      541 gtttctccgg tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt
      601 ctggaatacc agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac
      661 aactactaga ggaggccaac gccaagaagt ccacgctggt cggccacgac accttcacca
      721 aggtgaagcc gtccgccgcc tccggtggtg gatctgcgga ggtcggcatc accggcacct
      781 ggtacaacca gctcggctcg accttcatcg tgaccgcggg cgccgacggc gccctgaccg
      841 gaacctacga gtcggccgtc ggcaacgccg agagccgcta cgtcctgacc ggtcgttacg
      901 acagcgcccc ggccaccgac ggcagcggca ccgccctcgg ttggacggtg gcctggaaga
      961 ataactaccg caacgcccac tccgcgacca cgtggagcgg ccagtacgtc ggcggcgccg
     1021 aggcgaggat caacacccag tggctgctga cctccggcac cggctccgga accgccctcg
     1081 gttggacggt ggcctggaag aataactacc gcaacgccca ctccgcgacc acgtggagcg
     1141 gccagtacgt cggcggcgcc gaggcgagga tcaacaccca gtggctgctg acctccggca
     1201 ccaccgaggc caacgcctgg aagtccacgc tggtcggcca cgacaccttc accaaggtga
     1261 agccgtccgc cgcctccggt ggtggatctg cggaggtcgg catcaccggc acctggtaca
     1321 accagctcgg ctcgaccttc atcgtgaccg cgggcgccga cggcgccctg accggaacct
     1381 acgagtcggc cgtcggcaac gccgagagcc gctacgtcct gaccggtcgt tacgacagcg
     1441 ccccggccac cgacggcgcg gccgcactcg agcaccacca ccaccactga
//