LOCUS       BBa_J37015              2613 bp    DNA     linear       16-Jun-2024
DEFINITION  Prey Molecule Generator (AHL) + GFP Reporter
ACCESSION   BBa_J37015
VERSION     BBa_J37015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     misc_feature    1667..1698
                     /label=LVA
     CDS             1088..1666
                     /label=luxI
     misc_feature    1088..1705
                     /label=BBa_C0061
     misc_feature    1706..1730
                     /label=Help:Barcodes
     CDS             1088..1705
                     /label=BBa_C0061
     misc_feature    1743..1746
                     /label=conserved
     RBS             1739..1750
                     /label=BBa_B0034
     CDS             1757..2476
                     /label=GFP protein
     CDS             1757..2476
                     /label=BBa_E0040
     stem_loop       2496..2539
                     /label=stem_loop
     misc_feature    2485..2564
                     /label=BBa_B0010
     terminator      2485..2564
                     /label=BBa_B0010
     stem_loop       2580..2599
                     /label=T7 TE
     misc_feature    2606..2606
                     /label=stop
     polyA_site      2600..2613
                     /label=polya
     misc_feature    2573..2613
                     /label=BBa_B0012
     terminator      2573..2613
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta
     1141 taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt
     1201 tgtagaaaat aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc
     1261 ttgtgatgat actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta
     1321 tatgctgaaa agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa
     1381 tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc
     1441 tagtgaaatt acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat
     1501 tacagaatat gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt
     1561 tccttgtcat cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt
     1621 attgtctatg cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga
     1681 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa
     1741 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1801 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1861 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1921 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1981 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     2041 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     2101 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     2161 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     2221 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     2281 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     2341 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2401 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2461 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2521 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2581 gctcaccttc gggtgggcct ttctgcgttt ata
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