LOCUS       BBa_J37017              1965 bp    DNA     linear       12-May-2024
DEFINITION  AiiA Expression Assay (can be used in conjunction with AHL assay 
            to detect activity)
ACCESSION   BBa_J37017
VERSION     BBa_J37017.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1010..1015
                     /label=2
     misc_feature    977..1009
                     /label=LVA
     CDS             227..976
                     /label=aiiA
     misc_feature    227..1015
                     /label=BBa_C0060
     misc_feature    1016..1040
                     /label=Help:Barcodes
     CDS             227..1015
                     /label=BBa_C0060
     misc_feature    1053..1056
                     /label=conserved
     RBS             1049..1060
                     /label=BBa_B0034
     misc_feature    1823..1828
                     /label=stop
     misc_feature    1784..1822
                     /label=AAV
     misc_feature    1067..1828
                     /label=BBa_E0034
     stem_loop       1848..1891
                     /label=stem_loop
     misc_feature    1837..1916
                     /label=BBa_B0010
     terminator      1837..1916
                     /label=BBa_B0010
     stem_loop       1932..1951
                     /label=T7 TE
     misc_feature    1958..1958
                     /label=stop
     polyA_site      1952..1965
                     /label=polya
     misc_feature    1925..1965
                     /label=BBa_B0012
     terminator      1925..1965
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cagtaaagaa
      241 gctttatttc gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt
      301 aacaccagga gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg
      361 acctatttta gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa
      421 cggtacattt gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa
      481 tattttaaaa cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt
      541 gcattttgat catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg
      601 tgctgaatat gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa
      661 tttaaactac aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca
      721 tacaccaggc catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc
      781 tgtattatta acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt
      841 tgcgggattt gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat
      901 gaaagagaag ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt
      961 gttccctgaa tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg
     1021 atagtgctag tgtagatcgc tactagagaa agaggagaaa tactagatgg tgagcaaggg
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     1261 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1501 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     1801 cgaaaactac gctgctgctg tttaataata ctagagccag gcatcaaata aaacgaaagg
     1861 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1921 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//