LOCUS       BBa_J37020              1945 bp    DNA     linear       22-Dec-2024
DEFINITION  Predator Cell, pLux Transfer Function (two promoters)
ACCESSION   BBa_J37020
VERSION     BBa_J37020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     CDS             1089..1808
                     /label=GFP protein
     CDS             1089..1808
                     /label=BBa_E0040
     stem_loop       1828..1871
                     /label=stem_loop
     misc_feature    1817..1896
                     /label=BBa_B0010
     terminator      1817..1896
                     /label=BBa_B0010
     stem_loop       1912..1931
                     /label=T7 TE
     misc_feature    1938..1938
                     /label=stop
     polyA_site      1932..1945
                     /label=polya
     misc_feature    1905..1945
                     /label=BBa_B0012
     terminator      1905..1945
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1801 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1861 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1921 tcgggtgggc ctttctgcgt ttata
//