LOCUS       BBa_J37022              1181 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG induced AiiA w/ LVA and m-cyc tag
ACCESSION   BBa_J37022
VERSION     BBa_J37022.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    229..255
                     /label=Epitope Tag
     CDS             255..1005
                     /label=AiiA protein
     misc_feature    1005..1038
                     /label=Degredation Tag
     CDS             229..1044
                     /label=BBa_J37023
     stem_loop       1064..1107
                     /label=stem_loop
     misc_feature    1053..1132
                     /label=BBa_B0010
     terminator      1053..1132
                     /label=BBa_B0010
     stem_loop       1148..1167
                     /label=T7 TE
     misc_feature    1174..1174
                     /label=stop
     polyA_site      1168..1181
                     /label=polya
     misc_feature    1141..1181
                     /label=BBa_B0012
     terminator      1141..1181
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagga ttataaagat
      241 gatgatgata aaggtatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg
      301 ttggatcatt cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt
      361 tggtgttatc ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa
      421 agtgcagtta ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg
      481 aaaatgactg aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa
      541 gaccttcttt atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct
      601 tttataaata caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa
      661 gaatatttga aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat
      721 gaagtcgtac caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg
      781 ctattaattg agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg
      841 aaagagaatt ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct
      901 tcaattaaac gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat
      961 gatatagagc aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa
     1021 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca
     1081 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1141 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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