BBa_J37023 1 BBa_J37023 Immunologically Tagged aiiA 2006-07-26T11:00:00Z 2015-08-31T04:08:48Z [http://partsregistry.org/wiki/index.php/Part:BBa_C0060] This part is identical to the part BBa_C0060 [http://partsregistry.org/wiki/index.php/Part:BBa_C0060] but we have added an epitope tag to the protein. [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Spotlights/Antibodies_To_Epitope_Tags.html] This should allow us to accurately mesure the concentration of this protein using ELISA assays and purify and handle the protein. This is a work in progress and more data will be added when it is finished false false _66_ 0 1068 66 Not in stock false The FLAG had to be added at the 5' end of the sequence this was done using PCR false John Chattaway annotation1891625 1 Epitope Tag range1891625 1 1 27 annotation1891627 1 Degredation Tag range1891627 1 777 810 annotation1891626 1 AiiA protein range1891626 1 27 777 BBa_J37023_sequence 1 gattataaagatgatgatgataaaggtatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z