LOCUS       BBa_J37030              1395 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG -> Cre recombinase
ACCESSION   BBa_J37030
VERSION     BBa_J37030.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1258
                     /label=Cre recombinase
     CDS             227..1258
                     /label=BBa_J37029
     stem_loop       1278..1321
                     /label=stem_loop
     misc_feature    1267..1346
                     /label=BBa_B0010
     terminator      1267..1346
                     /label=BBa_B0010
     stem_loop       1362..1381
                     /label=T7 TE
     misc_feature    1388..1388
                     /label=stop
     polyA_site      1382..1395
                     /label=polya
     misc_feature    1355..1395
                     /label=BBa_B0012
     terminator      1355..1395
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccaatttact
      241 gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa
      301 gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct
      361 tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc
      421 cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt
      481 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc
      541 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt
      601 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt
      661 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg
      721 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat
      781 ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag
      841 caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat
      901 ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa
      961 tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt
     1021 tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc
     1081 ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc
     1141 aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat
     1201 ccgtaccctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattagta
     1261 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1321 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1381 ctttctgcgt ttata
//