LOCUS       BBa_J37035              2043 bp    DNA     linear       23-Dec-2024
DEFINITION  pLux controlled LuxR & AiiA
ACCESSION   BBa_J37035
VERSION     BBa_J37035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_J37018
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     misc_feature    1091..1117
                     /label=Epitope Tag
     CDS             1117..1867
                     /label=AiiA protein
     misc_feature    1867..1900
                     /label=Degredation Tag
     CDS             1091..1906
                     /label=BBa_J37023
     stem_loop       1926..1969
                     /label=stem_loop
     misc_feature    1915..1994
                     /label=BBa_B0010
     terminator      1915..1994
                     /label=BBa_B0010
     stem_loop       2010..2029
                     /label=T7 TE
     misc_feature    2036..2036
                     /label=stop
     polyA_site      2030..2043
                     /label=polya
     misc_feature    2003..2043
                     /label=BBa_B0012
     terminator      2003..2043
                     /label=BBa_B0012
     misc_feature    1071..2043
                     /label=BBa_J37025
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagag gattataaag atgatgatga taaaggtatg acagtaaaga agctttattt
     1141 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg
     1201 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt
     1261 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt
     1321 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa
     1381 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga
     1441 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata
     1501 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta
     1561 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg
     1621 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt
     1681 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt
     1741 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa
     1801 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga
     1861 atatatagct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc
     1921 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1981 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2041 ata
//