##gff-version 3
##sequence-region BBa_J37035 1 2043
BBa_J37035	.	engineered_region	1	1062	.	+	0	ID=BBa_J37018;Name=BBa_J37018
BBa_J37035	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37018
BBa_J37035	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J37035	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J37035	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J37035	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J37035	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J37035	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J37035	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J37018
BBa_J37035	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J37035	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J37035	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J37035	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J37035	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J37035	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J37018
BBa_J37035	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J37035	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J37035	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J37018
BBa_J37035	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J37035	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J37035	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J37035	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J37035	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J37035	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J37018
BBa_J37035	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J37035	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J37035	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J37035	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J37035	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37018
BBa_J37035	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J37035	.	engineered_region	1071	2043	.	+	0	ID=BBa_J37025;Name=BBa_J37025
BBa_J37035	.	ribosome_entry_site	1071	1082	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37025
BBa_J37035	.	conserved_region	1075	1078	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J37035	.	terminator	1915	1994	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J37025
BBa_J37035	.	stem_loop	1926	1969	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J37035	.	engineered_region	1915	1994	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J37035	.	terminator	2003	2043	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J37025
BBa_J37035	.	polyA_site	2030	2043	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J37035	.	stem_loop	2010	2029	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J37035	.	engineered_region	2003	2043	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J37035	.	stop_codon	2036	2036	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J37035	.	CDS	1091	1906	.	+	0	ID=BBa_J37023;Name=BBa_J37023;Parent=BBa_J37025
BBa_J37035	.	tag	1091	1117	.	+	0	ID=annotation1891625;Name=Epitope Tag;Parent=BBa_J37023
BBa_J37035	.	sequence_feature	1867	1900	.	+	0	ID=annotation1891627;Name=Degredation Tag;Parent=BBa_J37023
BBa_J37035	.	CDS	1117	1867	.	+	0	ID=annotation1891626;Name=AiiA protein;Parent=BBa_J37023
>BBa_J37035
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagaggattataaagatgatg
atgataaaggtatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatag
tacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagta
gatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccga
aaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattag
ttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatat
gaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgatt
atgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacaga
aaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcggga
tttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttg
gacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagt
agcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata