LOCUS       BBa_J37036              1843 bp    DNA     linear       01-Apr-2025
DEFINITION  Predator Molecule Generator (pLux expressing LuxR & AiiA)
ACCESSION   BBa_J37036
VERSION     BBa_J37036.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    1..862
                     /label=BBa_J37019
     misc_feature    875..878
                     /label=conserved
     RBS             871..882
                     /label=BBa_B0034
     misc_feature    891..917
                     /label=Epitope Tag
     CDS             917..1667
                     /label=AiiA protein
     misc_feature    1667..1700
                     /label=Degredation Tag
     CDS             891..1706
                     /label=BBa_J37023
     stem_loop       1726..1769
                     /label=stem_loop
     misc_feature    1715..1794
                     /label=BBa_B0010
     terminator      1715..1794
                     /label=BBa_B0010
     stem_loop       1810..1829
                     /label=T7 TE
     misc_feature    1836..1836
                     /label=stop
     polyA_site      1830..1843
                     /label=polya
     misc_feature    1803..1843
                     /label=BBa_B0012
     terminator      1803..1843
                     /label=BBa_B0012
     misc_feature    871..1843
                     /label=BBa_J37025
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagag gattataaag
      901 atgatgatga taaaggtatg acagtaaaga agctttattt cgtcccagca ggtcgttgta
      961 tgttggatca ttcgtctgtt aatagtacat taacaccagg agaattatta gacttaccgg
     1021 tttggtgtta tcttttggag actgaagaag gacctatttt agtagataca ggtatgccag
     1081 aaagtgcagt taataatgaa ggtcttttta acggtacatt tgtcgaaggg caggttttac
     1141 cgaaaatgac tgaagaagat agaatcgtga atattttaaa acgggttggt tatgagccgg
     1201 aagaccttct ttatattatt agttctcact tgcattttga tcatgcagga ggaaatggcg
     1261 cttttataaa tacaccaatc attgtacagc gtgctgaata tgaggcggcg cagcatagcg
     1321 aagaatattt gaaagaatgt atattgccga atttaaacta caaaatcatt gaaggtgatt
     1381 atgaagtcgt accaggagtt caattattgc atacaccagg ccatactcca gggcatcaat
     1441 cgctattaat tgagacagaa aaatccggtc ctgtattatt aacgattgat gcatcgtata
     1501 cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa ttagctttat
     1561 cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt ttctttggac
     1621 atgatataga gcaggaaagg ggatgtaaag tgttccctga atatatagct gcaaacgacg
     1681 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct
     1741 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1801 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//