LOCUS BBa_J37038 1349 bp DNA linear 22-Nov-2024 DEFINITION This device is to test the Pops Blocker part ACCESSION BBa_J37038 VERSION BBa_J37038.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 stem_loop 133..176 /label=stem_loop misc_feature 122..201 /label=BBa_B0010 terminator 122..201 /label=BBa_B0010 stem_loop 217..236 /label=T7 TE misc_feature 243..243 /label=stop polyA_site 237..250 /label=polya misc_feature 210..250 /label=BBa_B0012 terminator 210..250 /label=BBa_B0012 misc_feature 86..119 /label=BBa_J37026 misc_feature 122..201 /label=BBa_B0010 stem_loop 133..176 /label=stem_loop misc_feature 210..250 /label=BBa_B0012 stem_loop 217..236 /label=T7 TE polyA_site 237..250 /label=polya misc_feature 243..243 /label=stop misc_feature 1179..1212 /label=BBa_J37028 misc_feature 253..1176 /label=Reversed and complementary to part I13521 misc_feature 86..1212 /label=BBa_J37027 stem_loop 1232..1275 /label=stem_loop misc_feature 1221..1300 /label=BBa_B0010 terminator 1221..1300 /label=BBa_B0010 stem_loop 1316..1335 /label=T7 TE misc_feature 1342..1342 /label=stop polyA_site 1336..1349 /label=polya misc_feature 1309..1349 /label=BBa_B0012 terminator 1309..1349 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagagcgata acttggtata gcatacatta tacgaacggt 121 accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 181 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 241 tgcgtttata gattataaac gcagaaaggc ccacccgaag gtgagccagt gtgactctag 301 tagagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc 361 tttcgtttta tttgatgcct ggctctagta gcgatctaca ctagcactat cagcgttatt 421 aagcaccggt ggagtgacga ccttcagcac gttcgtactg ttcaacgatg gtgtagtctt 481 cgttgtggga ggtgatgtcc agtttgatgt cggttttgta agcacccggc agctgaaccg 541 gttttttagc catgtaggtg gttttaactt cagcgtcgta gtgaccaccg tctttcagtt 601 tcagacgcat tttgatttca cctttcagag caccgtcttc cgggtacata cgttcggtgg 661 aagcttccca acccatggtt tttttctgca taaccggacc gtcggacggg aagttggtac 721 cacgcagttt aactttgtag atgaactcac cgtcttgcag ggaggagtcc tgggtaacgg 781 taacaacacc accgtcttcg aagttcataa cacgttccca tttgaaacct tccgggaagg 841 acagtttcag gtagtccggg atgtcagccg ggtgtttaac gtaagctttg gaaccgtact 901 ggaactgcgg ggacaggatg tcccaagcga acggcagcgg accacctttg gtaactttca 961 gtttagcggt ctgggtacct tcgtacggac gaccttcacc ttcaccttcg atttcgaact 1021 cgtgaccgtt aacggaacct tccatacgaa ctttgaaacg catgaactct ttgataacgt 1081 cttcggagga agccatctag tatttctcct ctttctctag tagtgctcag tatctctatc 1141 actgataggg atgtcaatct ctatcactga tagggagtta ccgttcgtat acgatacatt 1201 atacgaagtt attactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1261 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1321 accttcgggt gggcctttct gcgtttata //