BBa_J37038
type: ComponentDefinition
persistent identity: BBa J37038
display id: BBa_J37038
version: 1
was generated by: iGEM to SBOL conversion
description: This device is to test the Pops Blocker part
created: 2006-09-12T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: registry
owned by: james
owned by: myers
mutable description: This device is to test the Pops Blocker part J37027
discontinued: false
dominant: false
group u list: _66_
m user id: 0
owner id: 1068
owning group id: 66
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: John Chattaway
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0012
component: BBa_B0010
component: BBa_J37027
component: BBa_B0030
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J37027
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J37038_sequence/1

BBa_B0012
type: Component
persistent identity: component1901712
display id: component1901712
version: 1
top level: BBa_J37038
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1901710
display id: component1901710
version: 1
top level: BBa_J37038
definition: BBa_B0010
access: public

BBa_J37027
type: Component
persistent identity: component1901709
display id: component1901709
version: 1
top level: BBa_J37038
definition: BBa_J37027
access: public

BBa_B0012
type: Component
persistent identity: component1901703
display id: component1901703
version: 1
top level: BBa_J37038
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1901701
display id: component1901701
version: 1
top level: BBa_J37038
definition: BBa_B0010
access: public

BBa_B0030
type: Component
persistent identity: component1901698
display id: component1901698
version: 1
top level: BBa_J37038
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component1901693
display id: component1901693
version: 1
top level: BBa_J37038
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1901693
display id: annotation1901693
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901693/range1901693/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J37038/annotation1901693/range1901693/1
type: Range
persistent identity: range1901693
display id: range1901693
version: 1
top level: BBa_J37038
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1901698
display id: annotation1901698
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901698/range1901698/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J37038/annotation1901698/range1901698/1
type: Range
persistent identity: range1901698
display id: range1901698
version: 1
top level: BBa_J37038
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_J37027
type: SequenceAnnotation
persistent identity: annotation1901709
display id: annotation1901709
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901709/range1901709/1
component: BBa_J37027

https://synbiohub.org/public/igem/BBa_J37038/annotation1901709/range1901709/1
type: Range
persistent identity: range1901709
display id: range1901709
version: 1
top level: BBa_J37038
direction: direction/reverse
start: 86
end: 1212
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1901701
display id: annotation1901701
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901701/range1901701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J37038/annotation1901701/range1901701/1
type: Range
persistent identity: range1901701
display id: range1901701
version: 1
top level: BBa_J37038
direction: direction/forward
start: 122
end: 201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1901703
display id: annotation1901703
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901703/range1901703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J37038/annotation1901703/range1901703/1
type: Range
persistent identity: range1901703
display id: range1901703
version: 1
top level: BBa_J37038
direction: direction/forward
start: 210
end: 250
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1901710
display id: annotation1901710
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901710/range1901710/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J37038/annotation1901710/range1901710/1
type: Range
persistent identity: range1901710
display id: range1901710
version: 1
top level: BBa_J37038
direction: direction/forward
start: 1221
end: 1300
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1901712
display id: annotation1901712
version: 1
top level: BBa_J37038
location: https://synbiohub.org/public/igem/BBa_J37038/annotation1901712/range1901712/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J37038/annotation1901712/range1901712/1
type: Range
persistent identity: range1901712
display id: range1901712
version: 1
top level: BBa_J37038
direction: direction/forward
start: 1309
end: 1349
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J37027
type: ComponentDefinition
persistent identity: BBa J37027
display id: BBa_J37027
version: 1
was generated by: iGEM to SBOL conversion
description: Pops Blocker (Cre/Lox system)
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: From Regestry: BBa_B0015 BBa_I13521 Lox Sites: Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH
owned by: james
owned by: myers
mutable description: This part is designed to be placed downstream of a promoter and prevent any Pops from the Promoter passing through this part. It will do this until an accompanying Cre Recombinase plasmid becomes activated. Once the Cre recombinase is activated the enzyme produced will permanently cut a section of DNA from the plasmid containing this part. This short section of DNA contains stop codons so once these are removed the polymerase can pass through this part and transcribe downstream genes. This short section of DNA is degraded. This is useful if a component of the system must be grown up but not activated until a certain external stimulus is added such as a positive feedback loop. This part should be very efficient at preventing Pops passing through it. The Part also contains a RFP reporter which is transcribed in the 3???-5??? direction. This means that un-activated parts will fluoresce red and activated parts will not fluoresce. This allows you to see that the part is working in your system. It also allows you to observe the efficiency of activation of the part in your system. Because of the way Cre recombinase works the excised reporter will remain in a small plasmid and continue to be transcribed for a short time however this plasmid will not have an origin of replication so will not be copied and the fluorescent protein should stop being produced after around 15min The design uses mutated lox sites, lox66 and lox71, which will make the excision irreversible. Reference: Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH
discontinued: false
dominant: false
experience: experience/None
group u list: _66_
m user id: 0
owner id: 1068
owning group id: 66
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Used specific lox sites to make this irreversable
star: true
creator: John Chattaway
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J37026
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: Reversed and complementary to part I13521
sequence annotation: BBa_J37028
sequence: https://synbiohub.org/public/igem/BBa_J37027_sequence/1

BBa_J37026
type: SequenceAnnotation
persistent identity: annotation1893177
display id: annotation1893177
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893177/range1893177/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J37027/annotation1893177/range1893177/1
type: Range
persistent identity: range1893177
display id: range1893177
version: 1
top level: BBa_J37027
direction: direction/reverse
start: 1
end: 34
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1893178
display id: annotation1893178
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893178/range1893178/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J37027/annotation1893178/range1893178/1
type: Range
persistent identity: range1893178
display id: range1893178
version: 1
top level: BBa_J37027
direction: direction/forward
start: 37
end: 116
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1893179
display id: annotation1893179
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893179/range1893179/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J37027/annotation1893179/range1893179/1
type: Range
persistent identity: range1893179
display id: range1893179
version: 1
top level: BBa_J37027
direction: direction/forward
start: 48
end: 91
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1893180
display id: annotation1893180
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893180/range1893180/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J37027/annotation1893180/range1893180/1
type: Range
persistent identity: range1893180
display id: range1893180
version: 1
top level: BBa_J37027
direction: direction/forward
start: 125
end: 165
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1893181
display id: annotation1893181
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893181/range1893181/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J37027/annotation1893181/range1893181/1
type: Range
persistent identity: range1893181
display id: range1893181
version: 1
top level: BBa_J37027
direction: direction/forward
start: 132
end: 151
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1893182
display id: annotation1893182
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893182/range1893182/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_J37027/annotation1893182/range1893182/1
type: Range
persistent identity: range1893182
display id: range1893182
version: 1
top level: BBa_J37027
direction: direction/forward
start: 152
end: 165
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1893183
display id: annotation1893183
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893183/range1893183/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J37027/annotation1893183/range1893183/1
type: Range
persistent identity: range1893183
display id: range1893183
version: 1
top level: BBa_J37027
direction: direction/forward
start: 158
end: 158
orientation: inline

Reversed and complementary to part I13521
type: SequenceAnnotation
persistent identity: annotation1893188
display id: annotation1893188
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893188/range1893188/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J37027/annotation1893188/range1893188/1
type: Range
persistent identity: range1893188
display id: range1893188
version: 1
top level: BBa_J37027
direction: direction/forward
start: 168
end: 1091
orientation: inline

BBa_J37028
type: SequenceAnnotation
persistent identity: annotation1893184
display id: annotation1893184
version: 1
top level: BBa_J37027
location: https://synbiohub.org/public/igem/BBa_J37027/annotation1893184/range1893184/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J37027/annotation1893184/range1893184/1
type: Range
persistent identity: range1893184
display id: range1893184
version: 1
top level: BBa_J37027
direction: direction/reverse
start: 1094
end: 1127
orientation: inline

https://synbiohub.org/public/igem/BBa_J37038_sequence/1
type: Sequence
persistent identity: BBa J37038 sequence
display id: BBa_J37038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagcgataacttggtatagcatacattatacgaacggtaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagattataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttctctagtagtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggagttaccgttcgtatacgatacattatacgaagttattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J37027_sequence/1
type: Sequence
persistent identity: BBa J37027 sequence
display id: BBa_J37027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgataacttggtatagcatacattatacgaacggtaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagattataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttctctagtagtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggagttaccgttcgtatacgatacattatacgaagttat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z