##gff-version 3
##sequence-region BBa_J3901 1 1349
BBa_J3901	.	promoter	1	272	.	+	0	ID=BBa_J3902;Name=BBa_J3902
BBa_J3901	.	promoter	7	266	.	+	0	ID=annotation1908375;Name=promoter;Parent=BBa_J3902
BBa_J3901	.	terminator	1221	1349	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J3901	.	terminator	1309	1349	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J3901	.	stem_loop	1316	1335	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3901	.	stop_codon	1342	1342	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J3901	.	polyA_site	1336	1349	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3901	.	engineered_region	1309	1349	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3901	.	terminator	1221	1300	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J3901	.	stem_loop	1232	1275	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3901	.	engineered_region	1221	1300	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3901	.	promoter	281	480	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J3901	.	promoter	417	422	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J3901	.	CDS	281	368	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J3901	.	engineered_region	281	480	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J3901	.	promoter	441	446	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J3901	.	non_covalent_binding_site	446	480	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J3901	.	start_codon	453	453	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J3901	.	non_covalent_binding_site	369	406	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J3901	.	ribosome_entry_site	489	500	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J3901	.	conserved_region	493	496	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J3901	.	CDS	507	1212	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J3901	.	CDS	507	1181	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J3901	.	engineered_tag	1188	1212	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_J3901
tctagattatgaccatcgaggcgatcgcgagatgcatggatgaggtagccatgcattttaatgagcgcataaaaagatg
ttgcaaagcatcgcggtttgtattaaatagaacgtgtgggtattgttaacaacgcaacaacattggttaaaggtcgagg
ctaattggcatcgcgttgttgtggtttggtgttaccagcctggcaggaagaccgggcgcatgagcgtattttgtttatc
taatctagatatgcctgaaagcgcataccgctatgtactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagt
tcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccag
accgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggtt
ccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacg
tgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaa
gttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccg
aacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacga
cgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata