LOCUS       BBa_J40000              2159 bp    DNA     linear       26-Dec-2024
DEFINITION  Quorum sensing promoter with lac I and CFP
ACCESSION   BBa_J40000
VERSION     BBa_J40000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     misc_feature    1247..1250
                     /label=conserved
     RBS             1243..1254
                     /label=BBa_B0034
     CDS             1261..2022
                     /label=CFP (LVA)
     misc_feature    1979..2016
                     /label=SsrA
     misc_feature    2017..2022
                     /label=2
     misc_feature    1329..1329
                     /label=A
     misc_feature    1261..2022
                     /label=BBa_E0022
     misc_feature    1261..2022
                     /label=BBa_E0022
     stem_loop       2042..2085
                     /label=stem_loop
     misc_feature    2031..2110
                     /label=BBa_B0010
     terminator      2031..2110
                     /label=BBa_B0010
     stem_loop       2126..2145
                     /label=T7 TE
     misc_feature    2152..2152
                     /label=stop
     polyA_site      2146..2159
                     /label=polya
     misc_feature    2119..2159
                     /label=BBa_B0012
     terminator      2119..2159
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag
     1261 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1321 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1381 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1441 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1501 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1561 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1621 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1681 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
     1741 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1801 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1861 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1921 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg
     1981 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca
     2041 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2101 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//