BBa_J40004 1 BBa_J40004 Jun-BetaE-YFPN 2006-10-25T11:00:00Z 2015-08-31T04:08:48Z Jun-IgA beta provided by LA Fernandez, Department of Microbial Biotechnology, Madrid. The YFP N-terminus was inserted into this plasmid as a PCR fragment; sequence determined by Hu and Kerppola, Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol. 2003 May;21(5):539-45 This is a fusion of Jun to the beta-autotransporter domain of the immunoglobulin A (IgA) protease of Neisseria gonorrhoea (IgA beta) and to the N-terminus of yellow fluorescent protein (YFP). The IgA beta should target the protein to the cell surface, and the Jun should bind Fos. If the bound Fos contains a C-terminus of a fluorescent protein, fluorescence is expected to result. false false _87_ 0 554 87 Not in stock false The sequence contained an EcoRI site that was removed by converting it to a Mun I site using oligo-based mutagenesis. Three Pst I sites are still found within the gene and will be removed at a later date by site-specific mutagenesis. false Jay Nadeau annotation1906001 1 BetaE range1906001 1 719 1936 annotation1906000 1 Jun range1906000 1 1 226 annotation1905998 1 YFPN range1905998 1 227 687 BBa_J40004_sequence 1 atgaaatacctattgcctacggcagccgctggattgttattactcgcggcccagccggccatggcggaccggatcgcccggctcgaggaaaaagtgaaaaccttgaaagcgcaaaactccgagctggcgtccacggccaacatgctcagggaacaggtggcacagcttaaacagaaagtcatgaaccacgaggcctcgggggcccaattcagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccccgcgggtcgctgcggccgtattttcattggatgattatgatgcaaaagacaatagtgaatcatcaataggtaatttagctcgtgtaatacctagaatgggaagggagttaattaatgattatgaagaaatccccttggaggagttggaagatgaagcggaagaagaacgtcgccaagcaacgcaattccactccaaaagtcgtaaccgtagagctatatcatcggaaccatcatctgatgaagatgcatctgaatcggtttccacatcagacaaacaccctcaagataatacggaacttcatgaaaaagttgagacggcgggtttacaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttacttaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatctgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgcagcttactttgccaattatggcaaaggcggcgtgaatgtgggcggtaaatccttcgcctataaagcagataatcaacagcaatattcagcaggcgtcgcgttactgtaccgtaatgttacattaaacgtaaatggcagtattacaaaaggaaaacaattggaaaaacaaaaatccggacaaattaaaatacagattcgtttcta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z