LOCUS BBa_J40007 6430 bp DNA linear 20-Nov-2024 DEFINITION LacI (LVA) + ECFP (LVA) under luxpR promoter + Repressilator ACCESSION BBa_J40007 VERSION BBa_J40007.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 1171..1209 /label=LVA CDS 82..1209 /label=lacI-LVA misc_feature 82..1209 /label=BBa_C0012 misc_feature 1210..1234 /label=Help:Barcodes CDS 82..1209 /label=BBa_C0012 misc_feature 64..1234 /label=BBa_S0100 misc_feature 1247..1250 /label=conserved RBS 1243..1254 /label=BBa_B0034 CDS 1261..2022 /label=CFP (LVA) misc_feature 1979..2016 /label=SsrA misc_feature 2017..2022 /label=2 misc_feature 1329..1329 /label=A misc_feature 1261..2022 /label=BBa_E0022 misc_feature 1261..2022 /label=BBa_E0022 stem_loop 2042..2085 /label=stem_loop misc_feature 2031..2110 /label=BBa_B0010 terminator 2031..2110 /label=BBa_B0010 stem_loop 2126..2145 /label=T7 TE misc_feature 2152..2152 /label=stop polyA_site 2146..2159 /label=polya misc_feature 2119..2159 /label=BBa_B0012 terminator 2119..2159 /label=BBa_B0012 misc_feature 1243..2159 /label=BBa_E0422 misc_feature 2168..2186 /label=TetR 1 misc_feature 2168..2221 /label=BBa_R0040 promoter 2187..2192 /label=-35 misc_feature 2193..2211 /label=TetR 2 promoter 2210..2215 /label=-10 promoter 2168..2221 /label=BBa_R0040 misc_feature 2234..2237 /label=conserved RBS 2230..2241 /label=BBa_B0034 CDS 2251..2958 /label=cI lambda misc_feature 2959..2991 /label=LVA misc_feature 2998..3022 /label=Help:Barcodes CDS 2248..2997 /label=BBa_C0051 stem_loop 3042..3085 /label=stem_loop misc_feature 3031..3110 /label=BBa_B0010 terminator 3031..3110 /label=BBa_B0010 stem_loop 3126..3145 /label=T7 TE misc_feature 3152..3152 /label=stop polyA_site 3146..3159 /label=polya misc_feature 3119..3159 /label=BBa_B0012 terminator 3119..3159 /label=BBa_B0012 promoter 3205..3210 /label=-10 promoter 3182..3187 /label=-35 misc_feature 3192..3208 /label=OR1 misc_feature 3168..3184 /label=OR2 misc_feature 3168..3216 /label=BBa_R0051 promoter 3168..3216 /label=BBa_R0051 misc_feature 2230..3216 /label=BBa_Q04510 misc_feature 3229..3232 /label=conserved RBS 3225..3236 /label=BBa_B0034 misc_feature 4332..4370 /label=LVA CDS 3243..4370 /label=lacI-LVA misc_feature 3243..4370 /label=BBa_C0012 misc_feature 4371..4395 /label=Help:Barcodes CDS 3243..4370 /label=BBa_C0012 stem_loop 4415..4458 /label=stem_loop misc_feature 4404..4483 /label=BBa_B0010 terminator 4404..4483 /label=BBa_B0010 stem_loop 4499..4518 /label=T7 TE misc_feature 4525..4525 /label=stop polyA_site 4519..4532 /label=polya misc_feature 4492..4532 /label=BBa_B0012 terminator 4492..4532 /label=BBa_B0012 terminator 4404..4532 /label=BBa_B0015 misc_feature 4543..4559 /label=lac O1 promoter 4560..4565 /label=-35 misc_feature 4566..4582 /label=lac O1 promoter 4583..4588 /label=-10 misc_feature 4541..4594 /label=BBa_R0011 promoter 4541..4594 /label=BBa_R0011 misc_feature 3225..4595 /label=BBa_Q04121 misc_feature 4608..4611 /label=conserved RBS 4604..4615 /label=BBa_B0034 CDS 4625..5241 /label=tetR misc_feature 5242..5275 /label=SsrA misc_feature 5282..5306 /label=Help:Barcodes CDS 4622..5281 /label=BBa_C0040 stem_loop 5326..5369 /label=stem_loop misc_feature 5315..5394 /label=BBa_B0010 terminator 5315..5394 /label=BBa_B0010 stem_loop 5410..5429 /label=T7 TE misc_feature 5436..5436 /label=stop polyA_site 5430..5443 /label=polya misc_feature 5403..5443 /label=BBa_B0012 terminator 5403..5443 /label=BBa_B0012 misc_feature 5452..5470 /label=TetR 1 misc_feature 5452..5505 /label=BBa_R0040 promoter 5471..5476 /label=-35 misc_feature 5477..5495 /label=TetR 2 promoter 5494..5499 /label=-10 promoter 5452..5505 /label=BBa_R0040 misc_feature 4604..5505 /label=BBa_Q04400 misc_feature 5518..5521 /label=conserved RBS 5514..5525 /label=BBa_B0034 CDS 5532..6293 /label=YFP (LVA) misc_feature 6288..6293 /label=2 misc_feature 6249..6287 /label=SsrA misc_feature 5773..5773 /label=A (Q->L) misc_feature 5532..6293 /label=BBa_E0032 misc_feature 5532..6293 /label=BBa_E0032 stem_loop 6313..6356 /label=stem_loop misc_feature 6302..6381 /label=BBa_B0010 terminator 6302..6381 /label=BBa_B0010 stem_loop 6397..6416 /label=T7 TE misc_feature 6423..6423 /label=stop polyA_site 6417..6430 /label=polya misc_feature 6390..6430 /label=BBa_B0012 terminator 6390..6430 /label=BBa_B0012 misc_feature 5514..6430 /label=BBa_E0432 misc_feature 2168..6430 /label=BBa_I5610 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca 121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta 1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag 1261 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1321 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 1381 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 1441 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag 1501 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 1561 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 1621 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 1681 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac 1741 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 1801 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 1861 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 1921 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg 1981 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca 2041 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 2101 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 2161 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca 2221 ctactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt aacacaagag 2281 cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc 2341 ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta 2401 tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa 2461 gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg 2521 gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag 2581 gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta 2641 agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg 2701 accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct 2761 gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc 2821 ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac 2881 ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg 2941 cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc 3001 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 3061 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3121 acactggctc accttcgggt gggcctttct gcgtttatat actagagtaa caccgtgcgt 3181 gttgactatt ttacctctgg cggtgataat ggttgctact agagaaagag gagaaatact 3241 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 3301 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 3361 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 3421 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 3481 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 3541 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 3601 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 3661 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 3721 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 3781 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 3841 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 3901 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 3961 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 4021 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 4081 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 4141 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 4201 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 4261 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 4321 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 4381 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4441 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 4501 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat 4561 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaatacta 4621 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 4681 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 4741 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 4801 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 4861 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 4921 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 4981 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 5041 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 5101 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 5161 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 5221 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 5281 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 5341 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 5401 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 5461 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 5521 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 5581 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 5641 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 5701 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg 5761 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 5821 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 5881 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 5941 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 6001 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 6061 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 6121 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg 6181 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 6241 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 6301 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 6361 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 6421 tgcgtttata //