BBa_J42022 1 BBa_J42022 PolyLinker for Nested Landing Pad 2006-10-30T12:00:00Z 2015-08-31T04:08:48Z Plasmid comes from pBR322, the insert was a custom construct of two annealed oligonucleotides. This is the initial poly-linker used to construct nested landing pads. It contains half of the pBR322 plasmid combined with a 9 site linker (EcoRI, SacI, NotI, NdeI, ClaI, BamHI, SalI, XhoI, HindIII) true false _81_ 0 1129 81 Discontinued false Polylinker had to be set up in a manner to facilitate compatible adjacent double digests. Also, polylinker sites had to be completely unique to vector sequence. false Steve Selinsky igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z