BBa_J42043 1 BBa_J42043 araB complete recomb. plasmid 2006-10-30T12:00:00Z 2015-08-31T04:08:48Z The polylinker, as well as the front and rear end of the AraB homologous regions come from annealed oligonucleotides. The Kanamycine gene extracted, via PCR, from the pDK11 plasmid This plasmid contains the front and rear end of the AraB gene flanking either end of a polylinker. It also contains a kanamycin resistance gene which allows confirmation of homologous recombination onto the chromosome. false false _81_ 0 1129 81 Not in stock false The flanking homologous regions and drug resistance gene frame a six restriction site poly-linker. Adjacent restriction sites had to be chosen for compatible double digestion to facilitate proper insert orientation. false Steve Selinsky BBa_J42043_sequence 1 gaattcatggcgattgcaattggcctcgattttggcagtgattctgtgcgagctttggcggtggactgcgctaccggtgaagagatcgccaccagcgagctcgggtttattattttttcggcctaattattagaccgaaaaatataagagacctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaagaattaattcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccagcctaaatcggtcaccagcggtcacgtgcccgcggccgcgggcatatggggatcgatgggggatccggggtcgacgggctcgagtatcgccgctatcagcaatgggcgatgagcgccgaacaacactatcttccaacttccgccccggcacaggctgcccaggccgttgcgactctataaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z