LOCUS       BBa_J43000              2189 bp    DNA     linear       22-Dec-2024
DEFINITION  J45503.Q04400.E0430
ACCESSION   BBa_J43000
VERSION     BBa_J43000.1  
FEATURES             Location/Qualifiers
     promoter        1..393
                     /label=BBa_J45503
     misc_feature    406..409
                     /label=conserved
     RBS             402..413
                     /label=BBa_B0034
     CDS             423..1039
                     /label=tetR
     misc_feature    1040..1073
                     /label=SsrA
     misc_feature    1080..1104
                     /label=Help:Barcodes
     CDS             420..1079
                     /label=BBa_C0040
     stem_loop       1124..1167
                     /label=stem_loop
     misc_feature    1113..1192
                     /label=BBa_B0010
     terminator      1113..1192
                     /label=BBa_B0010
     stem_loop       1208..1227
                     /label=T7 TE
     misc_feature    1234..1234
                     /label=stop
     polyA_site      1228..1241
                     /label=polya
     misc_feature    1201..1241
                     /label=BBa_B0012
     terminator      1201..1241
                     /label=BBa_B0012
     misc_feature    1250..1268
                     /label=TetR 1
     misc_feature    1250..1303
                     /label=BBa_R0040
     promoter        1269..1274
                     /label=-35
     misc_feature    1275..1293
                     /label=TetR 2
     promoter        1292..1297
                     /label=-10
     promoter        1250..1303
                     /label=BBa_R0040
     misc_feature    402..1303
                     /label=BBa_Q04400
     misc_feature    1316..1319
                     /label=conserved
     RBS             1312..1323
                     /label=BBa_B0034
     CDS             1330..2052
                     /label=BBa_E0030
     stem_loop       2072..2115
                     /label=stem_loop
     misc_feature    2061..2140
                     /label=BBa_B0010
     terminator      2061..2140
                     /label=BBa_B0010
     stem_loop       2156..2175
                     /label=T7 TE
     misc_feature    2182..2182
                     /label=stop
     polyA_site      2176..2189
                     /label=polya
     misc_feature    2149..2189
                     /label=BBa_B0012
     terminator      2149..2189
                     /label=BBa_B0012
     misc_feature    1312..2189
                     /label=BBa_E0430
ORIGIN
        1 cgccgctatg gactggataa agagatggta atggatttct ttcgtgagaa taattcctgt
       61 tctacgttgc gcttttttat ggccggttat cgcctcgaaa attgatcaaa catacgtatt
      121 atcttgcttt aattaattac actaatgctt cttcccttcg ttttagcgcc ccgccgcagt
      181 atcatgatat cgataaccat aataaatgtg tggtaaatgg cgcatcgatc gcattattga
      241 ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac ggaataacta taaatgactg
      301 gagataacac cctcatccat tctcacggca ttaaccgtcg tgatttcatg aagctttgtg
      361 cagcattagc cgccaccatg gggttaagta gcatactaga gaaagaggag aaatactaga
      421 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      481 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      541 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      601 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      661 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
      721 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
      781 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
      841 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
      901 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
      961 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     1021 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1081 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
     1141 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1201 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt
     1261 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag
     1321 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1381 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1441 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1501 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac
     1561 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1621 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1681 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1741 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1801 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1861 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1921 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1981 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2041 tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2101 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2161 accttcgggt gggcctttct gcgtttata
//