LOCUS       BBa_J43001              2658 bp    DNA     linear       22-Dec-2024
DEFINITION  J45503.Q04121.E0430
ACCESSION   BBa_J43001
VERSION     BBa_J43001.1  
FEATURES             Location/Qualifiers
     promoter        1..393
                     /label=BBa_J45503
     misc_feature    406..409
                     /label=conserved
     RBS             402..413
                     /label=BBa_B0034
     misc_feature    1509..1547
                     /label=LVA
     CDS             420..1547
                     /label=lacI-LVA
     misc_feature    420..1547
                     /label=BBa_C0012
     misc_feature    1548..1572
                     /label=Help:Barcodes
     CDS             420..1547
                     /label=BBa_C0012
     stem_loop       1592..1635
                     /label=stem_loop
     misc_feature    1581..1660
                     /label=BBa_B0010
     terminator      1581..1660
                     /label=BBa_B0010
     stem_loop       1676..1695
                     /label=T7 TE
     misc_feature    1702..1702
                     /label=stop
     polyA_site      1696..1709
                     /label=polya
     misc_feature    1669..1709
                     /label=BBa_B0012
     terminator      1669..1709
                     /label=BBa_B0012
     terminator      1581..1709
                     /label=BBa_B0015
     misc_feature    1720..1736
                     /label=lac O1
     promoter        1737..1742
                     /label=-35
     misc_feature    1743..1759
                     /label=lac O1
     promoter        1760..1765
                     /label=-10
     misc_feature    1718..1771
                     /label=BBa_R0011
     promoter        1718..1771
                     /label=BBa_R0011
     misc_feature    402..1772
                     /label=BBa_Q04121
     misc_feature    1785..1788
                     /label=conserved
     RBS             1781..1792
                     /label=BBa_B0034
     CDS             1799..2521
                     /label=BBa_E0030
     stem_loop       2541..2584
                     /label=stem_loop
     misc_feature    2530..2609
                     /label=BBa_B0010
     terminator      2530..2609
                     /label=BBa_B0010
     stem_loop       2625..2644
                     /label=T7 TE
     misc_feature    2651..2651
                     /label=stop
     polyA_site      2645..2658
                     /label=polya
     misc_feature    2618..2658
                     /label=BBa_B0012
     terminator      2618..2658
                     /label=BBa_B0012
     misc_feature    1781..2658
                     /label=BBa_E0430
ORIGIN
        1 cgccgctatg gactggataa agagatggta atggatttct ttcgtgagaa taattcctgt
       61 tctacgttgc gcttttttat ggccggttat cgcctcgaaa attgatcaaa catacgtatt
      121 atcttgcttt aattaattac actaatgctt cttcccttcg ttttagcgcc ccgccgcagt
      181 atcatgatat cgataaccat aataaatgtg tggtaaatgg cgcatcgatc gcattattga
      241 ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac ggaataacta taaatgactg
      301 gagataacac cctcatccat tctcacggca ttaaccgtcg tgatttcatg aagctttgtg
      361 cagcattagc cgccaccatg gggttaagta gcatactaga gaaagaggag aaatactaga
      421 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      481 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      541 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      601 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
      661 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
      721 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
      781 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
      841 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
      901 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
      961 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
     1021 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
     1081 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
     1141 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     1201 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
     1261 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     1321 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     1381 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     1441 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1501 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct
     1561 agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1621 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1681 accttcgggt gggcctttct gcgtttatat actagagaat tgtgagcgga taacaattga
     1741 cattgtgagc ggataacaag atactgagca catactagag aaagaggaga aatactagat
     1801 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1861 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1921 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1981 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct
     2041 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2101 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2161 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2221 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2281 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2341 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2401 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2461 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata
     2521 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2581 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2641 ggcctttctg cgtttata
//