##gff-version 3
##sequence-region BBa_J43001 1 2658
BBa_J43001	.	engineered_region	1781	2658	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_J43001	.	terminator	2530	2609	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_J43001	.	engineered_region	2530	2609	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J43001	.	stem_loop	2541	2584	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J43001	.	CDS	1799	2521	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_J43001	.	ribosome_entry_site	1781	1792	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_J43001	.	conserved_region	1785	1788	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J43001	.	terminator	2618	2658	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_J43001	.	stop_codon	2651	2651	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J43001	.	polyA_site	2645	2658	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J43001	.	stem_loop	2625	2644	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J43001	.	engineered_region	2618	2658	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J43001	.	engineered_region	402	1772	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_J43001	.	terminator	1581	1709	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J43001	.	terminator	1581	1660	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J43001	.	engineered_region	1581	1660	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J43001	.	stem_loop	1592	1635	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J43001	.	terminator	1669	1709	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J43001	.	stop_codon	1702	1702	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J43001	.	polyA_site	1696	1709	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J43001	.	stem_loop	1676	1695	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J43001	.	engineered_region	1669	1709	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J43001	.	promoter	1718	1771	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J43001	.	promoter	1760	1765	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J43001	.	engineered_region	1718	1771	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J43001	.	operator	1720	1736	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J43001	.	promoter	1737	1742	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J43001	.	operator	1743	1759	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J43001	.	CDS	420	1547	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J43001	.	engineered_tag	1548	1572	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J43001	.	engineered_region	420	1547	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J43001	.	tag	1509	1547	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J43001	.	CDS	420	1547	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J43001	.	ribosome_entry_site	402	413	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J43001	.	conserved_region	406	409	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J43001	.	promoter	1	393	.	+	0	ID=BBa_J45503;Name=BBa_J45503
>BBa_J43001
cgccgctatggactggataaagagatggtaatggatttctttcgtgagaataattcctgttctacgttgcgctttttta
tggccggttatcgcctcgaaaattgatcaaacatacgtattatcttgctttaattaattacactaatgcttcttccctt
cgttttagcgccccgccgcagtatcatgatatcgataaccataataaatgtgtggtaaatggcgcatcgatcgcattat
tgattttgcgattgaggcaaaatatatgccaggtcttcgcaacggaataactataaatgactggagataacaccctcat
ccattctcacggcattaaccgtcgtgatttcatgaagctttgtgcagcattagccgccaccatggggttaagtagcata
ctagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct
cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat
ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacc
tccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtgg
tggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgg
gctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttattt
cttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatc
tggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctgg
ctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggtttt
caacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcg
caatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacag
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaatt
gacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata