##gff-version 3
##sequence-region BBa_J44005 1 2587
BBa_J44005	.	sequence_feature	1120	1145	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J44005	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J44005	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J44005	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J44005	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J44005	.	CDS	230	802	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_J44005	.	primer_binding_site	230	232	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_J44005	.	sequence_alteration	299	304	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_J44005	.	primer_binding_site	780	799	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_J44005	.	sequence_alteration	301	301	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_J44005	.	CDS	230	802	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_J44005	.	terminator	899	939	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J44005	.	polyA_site	926	939	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J44005	.	engineered_region	899	939	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J44005	.	stem_loop	906	925	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J44005	.	stop_codon	932	932	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J44005	.	ribosome_entry_site	1154	1168	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J44005	.	ribosome_entry_site	1161	1165	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J44005	.	ribosome_entry_site	1154	1168	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J44005	.	engineered_region	1154	1168	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J44005	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J44005	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J44005	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J44005	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J44005	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J44005	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J44005	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J44005	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J44005	.	terminator	811	890	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J44005	.	engineered_region	811	890	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J44005	.	stem_loop	822	865	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J44005	.	terminator	2462	2502	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J44005	.	polyA_site	2489	2502	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J44005	.	engineered_region	2462	2502	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J44005	.	stem_loop	2469	2488	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J44005	.	stop_codon	2495	2495	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J44005	.	CDS	1175	2365	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_J44005	.	CDS	1175	2365	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_J44005	.	primer_binding_site	1175	1198	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_J44005	.	primer_binding_site	2346	2365	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_J44005	.	stop_codon	2363	2365	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_J44005	.	sequence_feature	948	973	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J44005	.	terminator	2374	2453	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J44005	.	engineered_region	2374	2453	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J44005	.	stem_loop	2385	2428	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J44005	.	sequence_feature	2511	2587	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_J44005	.	sequence_feature	2561	2561	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_J44005	.	non_covalent_binding_site	2565	2579	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_J44005	.	sequence_feature	2561	2566	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_J44005	.	sequence_feature	2539	2539	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_J44005	.	sequence_feature	2587	2587	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_J44005	.	sequence_feature	2511	2587	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_J44005	.	non_covalent_binding_site	2516	2531	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_J44005	.	promoter	982	1111	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_J44005
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctac
tattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgac
cgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaag
gcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattaca
tgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgta
atgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaag
gacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcc
tcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaa
cgaatgaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagt
tatcaaaaaccatggtttttgataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatt
tttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttggg
ctagctactagagttatcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaa
caatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggc
ctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaat
ttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagc
cactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcacc
ggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctca
tgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcacc
attccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagag
cgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcac
ttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctt
tcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcact
ggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgc
tggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgtt
gcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagccta
acttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattg
taggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattg
accaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg