BBa_J44005
type: ComponentDefinition
persistent identity: BBa J44005
display id: BBa_J44005
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
created: 2006-08-21T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: (BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_I13453), (BBa_J31007), (BBa_J3101)- from registry.
owned by: james
owned by: myers
mutable description: This a device that uses Hin to produce Hin recombinase that can flip the promotor pBAD for Tet resistance; this device can turn on/off the genes for Tet resistance.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Read-thru of the pBAD during transcription.
star: false
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J31000
component: BBa_J31007
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_J44000
component: BBa_I13453
component: BBa_J3101
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J44000
sequence annotation: BBa_I13453
sequence annotation: BBa_J31007
sequence annotation: BBa_J3101
sequence: https://synbiohub.org/public/igem/BBa_J44005_sequence/1

BBa_J31000
type: Component
persistent identity: component1897708
display id: component1897708
version: 1
top level: BBa_J44005
definition: Hin
access: public

BBa_J31007
type: Component
persistent identity: component1897722
display id: component1897722
version: 1
top level: BBa_J44005
definition: tetA(C)f
access: public

BBa_B0030
type: Component
persistent identity: component1897719
display id: component1897719
version: 1
top level: BBa_J44005
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component1897725
display id: component1897725
version: 1
top level: BBa_J44005
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1897723
display id: component1897723
version: 1
top level: BBa_J44005
definition: BBa_B0010
access: public

BBa_J44000
type: Component
persistent identity: component1897715
display id: component1897715
version: 1
top level: BBa_J44005
definition: hixC
access: public

BBa_B0012
type: Component
persistent identity: component1897711
display id: component1897711
version: 1
top level: BBa_J44005
definition: BBa_B0012
access: public

BBa_J44000
type: Component
persistent identity: component1897717
display id: component1897717
version: 1
top level: BBa_J44005
definition: hixC
access: public

BBa_I13453
type: Component
persistent identity: component1897716
display id: component1897716
version: 1
top level: BBa_J44005
definition: BBa_I13453
access: public

BBa_J3101
type: Component
persistent identity: component1897735
display id: component1897735
version: 1
top level: BBa_J44005
definition: RE
access: public

BBa_B0030
type: Component
persistent identity: component1897703
display id: component1897703
version: 1
top level: BBa_J44005
definition: BBa_B0030
access: public

BBa_R0010
type: Component
persistent identity: component1897695
display id: component1897695
version: 1
top level: BBa_J44005
definition: LacI
access: public

BBa_B0010
type: Component
persistent identity: component1897709
display id: component1897709
version: 1
top level: BBa_J44005
definition: BBa_B0010
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1897695
display id: annotation1897695
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897695/range1897695/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J44005/annotation1897695/range1897695/1
type: Range
persistent identity: range1897695
display id: range1897695
version: 1
top level: BBa_J44005
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1897703
display id: annotation1897703
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897703/range1897703/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J44005/annotation1897703/range1897703/1
type: Range
persistent identity: range1897703
display id: range1897703
version: 1
top level: BBa_J44005
direction: direction/forward
start: 209
end: 223
orientation: inline

BBa_J31000
type: SequenceAnnotation
persistent identity: annotation1897708
display id: annotation1897708
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897708/range1897708/1
component: BBa_J31000

https://synbiohub.org/public/igem/BBa_J44005/annotation1897708/range1897708/1
type: Range
persistent identity: range1897708
display id: range1897708
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 230
end: 802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1897709
display id: annotation1897709
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897709/range1897709/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J44005/annotation1897709/range1897709/1
type: Range
persistent identity: range1897709
display id: range1897709
version: 1
top level: BBa_J44005
direction: direction/forward
start: 811
end: 890
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1897711
display id: annotation1897711
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897711/range1897711/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J44005/annotation1897711/range1897711/1
type: Range
persistent identity: range1897711
display id: range1897711
version: 1
top level: BBa_J44005
direction: direction/forward
start: 899
end: 939
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1897715
display id: annotation1897715
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897715/range1897715/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44005/annotation1897715/range1897715/1
type: Range
persistent identity: range1897715
display id: range1897715
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 948
end: 973
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1897716
display id: annotation1897716
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897716/range1897716/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J44005/annotation1897716/range1897716/1
type: Range
persistent identity: range1897716
display id: range1897716
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 982
end: 1111
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1897717
display id: annotation1897717
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897717/range1897717/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44005/annotation1897717/range1897717/1
type: Range
persistent identity: range1897717
display id: range1897717
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 1120
end: 1145
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1897719
display id: annotation1897719
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897719/range1897719/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J44005/annotation1897719/range1897719/1
type: Range
persistent identity: range1897719
display id: range1897719
version: 1
top level: BBa_J44005
direction: direction/forward
start: 1154
end: 1168
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1897722
display id: annotation1897722
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897722/range1897722/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_J44005/annotation1897722/range1897722/1
type: Range
persistent identity: range1897722
display id: range1897722
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 1175
end: 2365
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1897723
display id: annotation1897723
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897723/range1897723/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J44005/annotation1897723/range1897723/1
type: Range
persistent identity: range1897723
display id: range1897723
version: 1
top level: BBa_J44005
direction: direction/forward
start: 2374
end: 2453
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1897725
display id: annotation1897725
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897725/range1897725/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J44005/annotation1897725/range1897725/1
type: Range
persistent identity: range1897725
display id: range1897725
version: 1
top level: BBa_J44005
direction: direction/forward
start: 2462
end: 2502
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation1897735
display id: annotation1897735
version: 1
top level: BBa_J44005
location: https://synbiohub.org/public/igem/BBa_J44005/annotation1897735/range1897735/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_J44005/annotation1897735/range1897735/1
type: Range
persistent identity: range1897735
display id: range1897735
version: 1
top level: BBa_J44005
direction: direction/reverse
start: 2511
end: 2587
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: iGEM to SBOL conversion
description: DNA-invertase Hin from Salmonella typhimurium
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium
owned by: myers
owned by: james
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Fwd primer J31001
sequence annotation: Hin sequence
sequence annotation: Former SpeI site
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Rev primer J31001
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 573
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
top level: Hin
direction: direction/forward
start: 70
end: 75
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
top level: Hin
direction: direction/forward
start: 72
end: 72
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
top level: Hin
direction: direction/reverse
start: 551
end: 570
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

RE
type: ComponentDefinition
persistent identity: BBa J3101
display id: BBa_J3101
version: 1
was generated by: iGEM to SBOL conversion
description: Recombinational Enhancer (RE) for Hin/Hix inverting
created: 2006-06-01T11:00:00Z
modified: 2015-08-31T04:08:45Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RE Sequence
sequence annotation: Proximal Fis Binding Site
sequence annotation: Originally a C
sequence annotation: Former SpeI site
sequence annotation: Mutation of SpeI site
sequence annotation: Distal Fis Binding Site
sequence annotation: Insertion right before biobrick ends
sequence: https://synbiohub.org/public/igem/BBa_J3101_sequence/1

RE Sequence
type: SequenceAnnotation
persistent identity: annotation1884988
display id: annotation1884988
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
type: Range
persistent identity: range1884988
display id: range1884988
version: 1
top level: RE
direction: direction/forward
start: 1
end: 77
orientation: inline

Proximal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884991
display id: annotation1884991
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
type: Range
persistent identity: range1884991
display id: range1884991
version: 1
top level: RE
direction: direction/forward
start: 6
end: 21
orientation: inline

Originally a C
type: SequenceAnnotation
persistent identity: annotation1884989
display id: annotation1884989
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
type: Range
persistent identity: range1884989
display id: range1884989
version: 1
top level: RE
direction: direction/forward
start: 29
end: 29
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880471
display id: annotation1880471
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
type: Range
persistent identity: range1880471
display id: range1880471
version: 1
top level: RE
direction: direction/forward
start: 51
end: 56
orientation: inline

Mutation of SpeI site
type: SequenceAnnotation
persistent identity: annotation1880472
display id: annotation1880472
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
type: Range
persistent identity: range1880472
display id: range1880472
version: 1
top level: RE
direction: direction/forward
start: 51
end: 51
orientation: inline

Distal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884992
display id: annotation1884992
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
type: Range
persistent identity: range1884992
display id: range1884992
version: 1
top level: RE
direction: direction/forward
start: 55
end: 69
orientation: inline

Insertion right before biobrick ends
type: SequenceAnnotation
persistent identity: annotation1884990
display id: annotation1884990
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
type: Range
persistent identity: range1884990
display id: range1884990
version: 1
top level: RE
direction: direction/forward
start: 77
end: 77
orientation: inline

https://synbiohub.org/public/igem/BBa_J44005_sequence/1
type: Sequence
persistent identity: BBa J44005 sequence
display id: BBa_J44005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31000_sequence/1
type: Sequence
persistent identity: BBa J31000 sequence
display id: BBa_J31000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J3101_sequence/1
type: Sequence
persistent identity: BBa J3101 sequence
display id: BBa_J3101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z