##gff-version 3
##sequence-region BBa_J44008 1 2626
BBa_J44008	.	sequence_feature	1159	1184	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J44008	.	CDS	1214	2404	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_J44008	.	primer_binding_site	1214	1237	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_J44008	.	CDS	1214	2404	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_J44008	.	stop_codon	2402	2404	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_J44008	.	primer_binding_site	2385	2404	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_J44008	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J44008	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J44008	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J44008	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J44008	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J44008	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J44008	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J44008	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J44008	.	ribosome_entry_site	1193	1207	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J44008	.	ribosome_entry_site	1200	1204	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J44008	.	ribosome_entry_site	1193	1207	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J44008	.	engineered_region	1193	1207	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J44008	.	sequence_feature	987	1012	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J44008	.	promoter	1021	1150	.	+	0	ID=BBa_J44002;Name=BBa_J44002
BBa_J44008	.	promoter	1021	1150	.	+	0	ID=annotation2002776;Name=promoter;Parent=BBa_J44002
BBa_J44008	.	terminator	2413	2541	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J44008	.	terminator	2501	2541	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J44008	.	polyA_site	2528	2541	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J44008	.	engineered_region	2501	2541	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J44008	.	stem_loop	2508	2527	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J44008	.	stop_codon	2534	2534	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J44008	.	terminator	2413	2492	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J44008	.	stem_loop	2424	2467	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J44008	.	engineered_region	2413	2492	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J44008	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J44008	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J44008	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J44008	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J44008	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J44008	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J44008	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J44008	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J44008	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J44008	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J44008	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J44008	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J44008	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J44008	.	CDS	230	841	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_J44008	.	primer_binding_site	230	232	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_J44008	.	primer_binding_site	780	841	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_J44008	.	tag	800	838	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_J44008	.	CDS	230	799	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_J44008	.	stop_codon	839	841	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_J44008	.	sequence_feature	2550	2626	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_J44008	.	sequence_feature	2550	2626	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_J44008	.	sequence_feature	2600	2600	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_J44008	.	sequence_feature	2578	2578	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_J44008	.	non_covalent_binding_site	2604	2618	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_J44008	.	non_covalent_binding_site	2555	2570	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_J44008	.	sequence_feature	2600	2605	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_J44008	.	sequence_feature	2626	2626	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
>BBa_J44008
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctac
tattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgac
cgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaag
gcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattaca
tgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgta
atgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaag
gacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcc
tcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaa
cgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataatactagaggctagcc
caaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaa
tgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagttatcaaaaaccatggtttttgataat
actagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggat
gctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtc
actatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctt
tggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctg
tggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgaca
tcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgt
ggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctacta
ctgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagct
ccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggt
gccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggta
ttcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccatta
tcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattat
gattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcag
ggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatg
ccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcg
tcgcggtgcatggagccgggccacctcgacctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgc
aaattgtgaccgcattttg