LOCUS       BBa_J44013              1454 bp    DNA     linear       17-May-2024
DEFINITION  HixC-pBAD-HixC-Tetrev-RBSrev-HixC
ACCESSION   BBa_J44013
VERSION     BBa_J44013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     promoter        35..164
                     /label=BBa_I13453
     misc_feature    173..198
                     /label=BBa_J44000
     misc_feature    35..198
                     /label=BBa_S03529
     CDS             207..1397
                     /label=tetA(C) backwards
     misc_feature    207..209
                     /label=stop
     primer_bind     207..226
                     /label=Rev primer J31006
     primer_bind     1374..1397
                     /label=Fwd primer J31006
     CDS             207..1397
                     /label=BBa_J31006
     RBS             1406..1420
                     /label=Reverse RBS
     RBS             1406..1420
                     /label=BBa_J44001
     misc_feature    207..1420
                     /label=BBa_S03532
     misc_feature    35..1420
                     /label=BBa_S03533
     misc_feature    1429..1454
                     /label=BBa_J44000
     misc_feature    35..1454
                     /label=BBa_S03624
     misc_feature    1..1454
                     /label=BBa_J44011
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagacattg attatttgca cggcgtcaca
       61 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
      121 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agttatcaaa
      181 aaccatggtt tttgataata ctagagttag gtcgaggtgg cccggctcca tgcaccgcga
      241 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
      301 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt
      361 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
      421 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
      481 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
      541 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc ggggccagtg
      601 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
      661 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
      721 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
      781 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc
      841 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
      901 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
      961 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     1021 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     1081 gccacgatgc gtccggcgta gaggatccac aggacgggtg tggtcgccat gatcgcgtag
     1141 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
     1201 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
     1261 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
     1321 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     1381 gcattgttag atttcattac tagagtttct cctctttaat tactagagtt atcaaaaacc
     1441 atggtttttg ataa
//