BBa_J44013
type: ComponentDefinition
persistent identity: BBa J44013
display id: BBa_J44013
version: 1
was generated by: iGEM to SBOL conversion
description: HixC-pBAD-HixC-Tetrev-RBSrev-HixC
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Cloned from J44011.
owned by: james
owned by: myers
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from reverse to forward orientation. Cloned in pSB1A7 - no readthrough transcription detected.
discontinued: false
dominant: true
experience: experience/None
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.
star: false
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J44011
sequence annotation: BBa_J44011
sequence: https://synbiohub.org/public/igem/BBa_J44013_sequence/1

BBa_J44011
type: Component
persistent identity: component2223909
display id: component2223909
version: 1
top level: BBa_J44013
definition: BBa_J44011
access: public

BBa_J44011
type: SequenceAnnotation
persistent identity: annotation2223909
display id: annotation2223909
version: 1
top level: BBa_J44013
location: https://synbiohub.org/public/igem/BBa_J44013/annotation2223909/range2223909/1
component: BBa_J44011

https://synbiohub.org/public/igem/BBa_J44013/annotation2223909/range2223909/1
type: Range
persistent identity: range2223909
display id: range2223909
version: 1
top level: BBa_J44013
direction: direction/reverse
start: 1
end: 1454
orientation: inline

BBa_J44011
type: ComponentDefinition
persistent identity: BBa J44011
display id: BBa_J44011
version: 1
was generated by: iGEM to SBOL conversion
description: HixC-pBAD-HixC-Tetrev-RBSrev-HixC
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: HixC is synthetic, rest is from part BBa_S03626.
owned by: james
owned by: myers
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from reverse to forward orientation.
discontinued: false
dominant: true
experience: experience/None
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_S03624
component: BBa_J44000
sequence annotation: BBa_J44000
sequence annotation: BBa_S03624
sequence: https://synbiohub.org/public/igem/BBa_J44011_sequence/1

BBa_S03624
type: Component
persistent identity: component2223892
display id: component2223892
version: 1
top level: BBa_J44011
definition: BBa_S03624
access: public

BBa_J44000
type: Component
persistent identity: component2223878
display id: component2223878
version: 1
top level: BBa_J44011
definition: hixC
access: public

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation2223878
display id: annotation2223878
version: 1
top level: BBa_J44011
location: https://synbiohub.org/public/igem/BBa_J44011/annotation2223878/range2223878/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44011/annotation2223878/range2223878/1
type: Range
persistent identity: range2223878
display id: range2223878
version: 1
top level: BBa_J44011
direction: direction/reverse
start: 1
end: 26
orientation: inline

BBa_S03624
type: SequenceAnnotation
persistent identity: annotation2223892
display id: annotation2223892
version: 1
top level: BBa_J44011
location: https://synbiohub.org/public/igem/BBa_J44011/annotation2223892/range2223892/1
component: BBa_S03624

https://synbiohub.org/public/igem/BBa_J44011/annotation2223892/range2223892/1
type: Range
persistent identity: range2223892
display id: range2223892
version: 1
top level: BBa_J44011
direction: direction/reverse
start: 35
end: 1454
orientation: inline

BBa_S03624
type: ComponentDefinition
persistent identity: BBa S03624
display id: BBa_S03624
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-HixC-TetB-RBSrev : HixC
created: 2006-10-25T11:00:00Z
modified: 2015-05-08T01:14:25Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _61_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S03533
component: BBa_J44000
sequence annotation: BBa_S03533
sequence annotation: BBa_J44000
sequence: https://synbiohub.org/public/igem/BBa_S03624_sequence/1

BBa_S03533
type: Component
persistent identity: component2221943
display id: component2221943
version: 1
top level: BBa_S03624
definition: BBa_S03533
access: public

BBa_J44000
type: Component
persistent identity: component2221944
display id: component2221944
version: 1
top level: BBa_S03624
definition: hixC
access: public

BBa_S03533
type: SequenceAnnotation
persistent identity: annotation2221943
display id: annotation2221943
version: 1
top level: BBa_S03624
location: https://synbiohub.org/public/igem/BBa_S03624/annotation2221943/range2221943/1
component: BBa_S03533

https://synbiohub.org/public/igem/BBa_S03624/annotation2221943/range2221943/1
type: Range
persistent identity: range2221943
display id: range2221943
version: 1
top level: BBa_S03624
direction: direction/reverse
start: 1
end: 1386
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation2221944
display id: annotation2221944
version: 1
top level: BBa_S03624
location: https://synbiohub.org/public/igem/BBa_S03624/annotation2221944/range2221944/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03624/annotation2221944/range2221944/1
type: Range
persistent identity: range2221944
display id: range2221944
version: 1
top level: BBa_S03624
direction: direction/reverse
start: 1395
end: 1420
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

BBa_S03533
type: ComponentDefinition
persistent identity: BBa S03533
display id: BBa_S03533
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-HixC-TetB-RBSrev
created: 2006-08-01T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S03532
component: BBa_S03529
sequence annotation: BBa_S03529
sequence annotation: BBa_S03532
sequence: https://synbiohub.org/public/igem/BBa_S03533_sequence/1

BBa_S03532
type: Component
persistent identity: component2220331
display id: component2220331
version: 1
top level: BBa_S03533
definition: BBa_S03532
access: public

BBa_S03529
type: Component
persistent identity: component2220323
display id: component2220323
version: 1
top level: BBa_S03533
definition: BBa_S03529
access: public

BBa_S03529
type: SequenceAnnotation
persistent identity: annotation2220323
display id: annotation2220323
version: 1
top level: BBa_S03533
location: https://synbiohub.org/public/igem/BBa_S03533/annotation2220323/range2220323/1
component: BBa_S03529

https://synbiohub.org/public/igem/BBa_S03533/annotation2220323/range2220323/1
type: Range
persistent identity: range2220323
display id: range2220323
version: 1
top level: BBa_S03533
direction: direction/reverse
start: 1
end: 164
orientation: inline

BBa_S03532
type: SequenceAnnotation
persistent identity: annotation2220331
display id: annotation2220331
version: 1
top level: BBa_S03533
location: https://synbiohub.org/public/igem/BBa_S03533/annotation2220331/range2220331/1
component: BBa_S03532

https://synbiohub.org/public/igem/BBa_S03533/annotation2220331/range2220331/1
type: Range
persistent identity: range2220331
display id: range2220331
version: 1
top level: BBa_S03533
direction: direction/reverse
start: 173
end: 1386
orientation: inline

BBa_S03529
type: ComponentDefinition
persistent identity: BBa S03529
display id: BBa_S03529
version: 1
was generated by: iGEM to SBOL conversion
description: pBad : HixC
created: 2006-07-31T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
discontinued: false
dominant: true
experience: experience/None
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I13453
component: BBa_J44000
sequence annotation: BBa_I13453
sequence annotation: BBa_J44000
sequence: https://synbiohub.org/public/igem/BBa_S03529_sequence/1

BBa_I13453
type: Component
persistent identity: component1892379
display id: component1892379
version: 1
top level: BBa_S03529
definition: BBa_I13453
access: public

BBa_J44000
type: Component
persistent identity: component1892380
display id: component1892380
version: 1
top level: BBa_S03529
definition: hixC
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1892379
display id: annotation1892379
version: 1
top level: BBa_S03529
location: https://synbiohub.org/public/igem/BBa_S03529/annotation1892379/range1892379/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_S03529/annotation1892379/range1892379/1
type: Range
persistent identity: range1892379
display id: range1892379
version: 1
top level: BBa_S03529
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892380
display id: annotation1892380
version: 1
top level: BBa_S03529
location: https://synbiohub.org/public/igem/BBa_S03529/annotation1892380/range1892380/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03529/annotation1892380/range1892380/1
type: Range
persistent identity: range1892380
display id: range1892380
version: 1
top level: BBa_S03529
direction: direction/reverse
start: 139
end: 164
orientation: inline

BBa_S03532
type: ComponentDefinition
persistent identity: BBa S03532
display id: BBa_S03532
version: 1
was generated by: iGEM to SBOL conversion
description: TetB-RBSrev
created: 2006-08-01T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J44001
component: BBa_J31006
sequence annotation: BBa_J31006
sequence annotation: BBa_J44001
sequence: https://synbiohub.org/public/igem/BBa_S03532_sequence/1

BBa_J44001
type: Component
persistent identity: component1932652
display id: component1932652
version: 1
top level: BBa_S03532
definition: BBa_J44001
access: public

BBa_J31006
type: Component
persistent identity: component1932650
display id: component1932650
version: 1
top level: BBa_S03532
definition: tetA(C)b
access: public

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation1932650
display id: annotation1932650
version: 1
top level: BBa_S03532
location: https://synbiohub.org/public/igem/BBa_S03532/annotation1932650/range1932650/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_S03532/annotation1932650/range1932650/1
type: Range
persistent identity: range1932650
display id: range1932650
version: 1
top level: BBa_S03532
direction: direction/reverse
start: 1
end: 1191
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation1932652
display id: annotation1932652
version: 1
top level: BBa_S03532
location: https://synbiohub.org/public/igem/BBa_S03532/annotation1932652/range1932652/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_S03532/annotation1932652/range1932652/1
type: Range
persistent identity: range1932652
display id: range1932652
version: 1
top level: BBa_S03532
direction: direction/reverse
start: 1200
end: 1214
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

https://synbiohub.org/public/igem/BBa_J44013_sequence/1
type: Sequence
persistent identity: BBa J44013 sequence
display id: BBa_J44013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44011_sequence/1
type: Sequence
persistent identity: BBa J44011 sequence
display id: BBa_J44011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03624_sequence/1
type: Sequence
persistent identity: BBa S03624 sequence
display id: BBa_S03624_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03533_sequence/1
type: Sequence
persistent identity: BBa S03533 sequence
display id: BBa_S03533_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03529_sequence/1
type: Sequence
persistent identity: BBa S03529 sequence
display id: BBa_S03529_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03532_sequence/1
type: Sequence
persistent identity: BBa S03532 sequence
display id: BBa_S03532_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z