LOCUS       BBa_J44014              1261 bp    DNA     linear       19-Apr-2025
DEFINITION  T7 promoter - RBS - RFP1 - hixC - RFP2
ACCESSION   BBa_J44014
VERSION     BBa_J44014.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    524..527
                     /label=conserved
     RBS             520..531
                     /label=BBa_B0034
     misc_feature    538..540
                     /label=start
     CDS             538..999
                     /label=Coding sequence
     CDS             538..999
                     /label=BBa_I715022
     misc_feature    1008..1033
                     /label=BBa_J44000
     misc_feature    1042..1042
                     /label=extra base (avoid frameshift)
     CDS             1043..1255
                     /label=Continue coding sequence
     misc_feature    1256..1260
                     /label=(two in tandem)
     CDS             1042..1261
                     /label=BBa_I715023
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagaga aagaggagaa atactagatg
      541 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
      601 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
      661 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
      721 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
      781 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
      841 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
      901 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
      961 ggttgggaag cttccaccga acgtatgtac ccggaagact actagagtta tcaaaaacca
     1021 tggtttttga taatactaga gtggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
     1081 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
     1141 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
     1201 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
     1261 a
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