LOCUS       BBa_J44015               773 bp    DNA     linear       26-Dec-2024
DEFINITION  T7 promoter - RBS - RFP1 - hixC - RFP2
ACCESSION   BBa_J44015
VERSION     BBa_J44015.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base (avoid frameshift)
     CDS             555..767
                     /label=Continue coding sequence
     misc_feature    768..772
                     /label=(two in tandem)
     CDS             554..773
                     /label=BBa_I715023
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taa
//
LOCUS       BBa_S03798               773 bp    DNA     linear       26-Dec-2024
DEFINITION  T7-RBS-RFP1-hixC-RFP2
ACCESSION   BBa_S03798
VERSION     BBa_S03798.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base (avoid frameshift)
     CDS             555..767
                     /label=Continue coding sequence
     misc_feature    768..772
                     /label=(two in tandem)
     CDS             554..773
                     /label=BBa_I715023
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taa
//