##gff-version 3
##sequence-region BBa_J45009 1 1830
BBa_J45009	.	start_codon	1	3	.	+	0	ID=annotation1961303;Name=start codon
BBa_J45009	.	point_mutation	699	699	.	+	0	ID=annotation1891632;Name=t->c to eliminate SpeI, to do
BBa_J45009	.	CDS	1	1830	.	+	0	ID=annotation1961298;Name=THI3
BBa_J45009	.	point_mutation	1791	1791	.	+	0	ID=annotation1891631;Name=a->g to eliminate EcoRI, to do
BBa_J45009	.	point_mutation	6	6	.	+	0	ID=annotation1891628;Name=t->c to eliminate EcoRI, to do
BBa_J45009	.	stop_codon	1828	1830	.	+	0	ID=annotation1961304;Name=stop codon
BBa_J45009	.	point_mutation	1605	1605	.	+	0	ID=annotation1891630;Name=a->g to eliminate EcoRI, to do
BBa_J45009	.	point_mutation	906	906	.	+	0	ID=annotation1891629;Name=a->g to eliminate EcoRI, to do
>BBa_J45009
atgaattctagctatacacagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaacc
agctgaacatacataccatatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaa
cttacgatgggccggtaattctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttggga
tgtctcataacaacctttggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaa
tacttcacatagtgggtatgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtga
tttcacggtatttcatagaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgac
gaagtcgataagtgcatcaaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatc
tcccgattgaatcagcaaggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagt
tatttctcgaatattgagttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaat
ttaatcgaggagactaaagagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaa
acgaaacagacccgcaatttgggggcgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgc
cgattttatcatcgtcattggttgcatgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaat
tgtgcgctactatattctacatctgtgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacaga
aaatattagcaaatcttgatgaatctaaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagacctta
cccagcaggaaatgtcctcttgagacaagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatc
ataacagaaactggtgcttctgcatttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctc
tttggggatctgtcggatatacaatgggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaatt
ccccgcaactaaacatagagttattctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaatt
gttaagtggggattgacaccttatatttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggt
cagatgctagttattacgatatccaaccttggaactacttgggattattgcgagtatttggttgcacgaactacgaaac
gaaaaaaattattactgttggagaattcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatg
atagagattatgctaccaccaagggatgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaac
aagtgcaagaggagaacgaaaattctagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatca
agttggatactga