##gff-version 3
##sequence-region BBa_J45120 1 1292
BBa_J45120	.	terminator	1164	1243	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45120	.	stem_loop	1175	1218	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45120	.	engineered_region	1164	1243	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45120	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45120	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45120	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45120	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45120	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45120	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45120	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45120	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45120	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45120	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45120	.	terminator	1252	1292	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45120	.	polyA_site	1279	1292	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45120	.	stem_loop	1259	1278	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45120	.	engineered_region	1252	1292	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45120	.	stop_codon	1285	1285	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45120	.	CDS	82	1155	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_J45120	.	stop_codon	1153	1155	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45120	.	start_codon	82	84	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45120	.	CDS	82	1155	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
>BBa_J45120
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagca
aaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaacctta
tgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaag
aacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacact
ttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtgga
gtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggc
tatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaa
agcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaag
atggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagcca
tggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacacc
atcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcat
tggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttg
tcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaaga
gaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata