##gff-version 3
##sequence-region BBa_J45150 1 1297
BBa_J45150	.	terminator	1257	1297	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45150	.	polyA_site	1284	1297	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45150	.	engineered_region	1257	1297	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45150	.	stem_loop	1264	1283	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45150	.	stop_codon	1290	1290	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45150	.	ribosome_entry_site	66	80	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45150	.	ribosome_entry_site	73	77	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45150	.	ribosome_entry_site	66	80	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45150	.	engineered_region	66	80	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45150	.	promoter	1	57	.	+	0	ID=BBa_J45993;Name=BBa_J45993
BBa_J45150	.	promoter	22	27	.	+	0	ID=annotation1883648;Name=-35;Parent=BBa_J45993
BBa_J45150	.	promoter	46	51	.	+	0	ID=annotation1883647;Name=-10;Parent=BBa_J45993
BBa_J45150	.	CDS	87	1160	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_J45150	.	stop_codon	1158	1160	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45150	.	CDS	87	1160	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45150	.	start_codon	87	89	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45150	.	terminator	1169	1248	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45150	.	engineered_region	1169	1248	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45150	.	stem_loop	1180	1223	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J45150
gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaa
atactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggtt
cagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaa
ccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtaga
aaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaat
acactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttca
gtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcat
gtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgtt
attaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtg
gaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttct
agccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatac
acaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagag
ttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagccttt
gcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtcc
aaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata