LOCUS       BBa_J45180              2205 bp    DNA     linear       02-May-2025
DEFINITION  Exponential phase dependent wintergreen odor generator
ACCESSION   BBa_J45180
VERSION     BBa_J45180.1  
FEATURES             Location/Qualifiers
     promoter        46..51
                     /label=-10
     promoter        22..27
                     /label=-35
     promoter        1..57
                     /label=BBa_J45993
     RBS             66..77
                     /label=BBa_B0064
     CDS             87..703
                     /label=tetR
     misc_feature    704..737
                     /label=SsrA
     misc_feature    744..768
                     /label=Help:Barcodes
     CDS             84..768
                     /label=BBa_C0040
     stem_loop       788..831
                     /label=stem_loop
     misc_feature    777..856
                     /label=BBa_B0010
     terminator      777..856
                     /label=BBa_B0010
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     terminator      777..905
                     /label=BBa_B0015
     misc_feature    914..932
                     /label=TetR 1
     misc_feature    914..967
                     /label=BBa_R0040
     promoter        933..938
                     /label=-35
     misc_feature    939..957
                     /label=TetR 2
     promoter        956..961
                     /label=-10
     promoter        914..967
                     /label=BBa_R0040
     misc_feature    66..967
                     /label=BBa_Q04401
     RBS             976..988
                     /label=RBS-3\Weak
     RBS             982..985
                     /label=RBS
     misc_feature    976..988
                     /label=BBa_B0032
     RBS             976..988
                     /label=BBa_B0032
     CDS             995..2068
                     /label=BMST1
     misc_feature    995..997
                     /label=start codon
     misc_feature    2066..2068
                     /label=stop codon
     CDS             995..2068
                     /label=BBa_J45004
     stem_loop       2088..2131
                     /label=stem_loop
     misc_feature    2077..2156
                     /label=BBa_B0010
     terminator      2077..2156
                     /label=BBa_B0010
     stem_loop       2172..2191
                     /label=T7 TE
     misc_feature    2198..2198
                     /label=stop
     polyA_site      2192..2205
                     /label=polya
     misc_feature    2165..2205
                     /label=BBa_B0012
     terminator      2165..2205
                     /label=BBa_B0012
ORIGIN
        1 gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat ttaacaatac
       61 tagagaaaga ggggaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      301 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      721 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      961 tgagcactac tagagtcaca caggaaagta ctagatggaa gttgttgaag ttcttcacat
     1021 gaatggagga aatggagaca gtagctatgc aaacaattct ttggttcagc aaaaggtgat
     1081 tctcatgaca aagccaataa ctgagcaagc catgattgat ctctacagca gcctctttcc
     1141 agaaacctta tgcattgcag atttgggttg ttctttggga gctaacactt tcttggtggt
     1201 ctcacagctt gttaaaatag tagaaaaaga acgaaaaaag catggtttta agtctccaga
     1261 gttttatttt cacttcaatg atcttcctgg caatgatttt aatacacttt ttcagtcact
     1321 gggggcattt caagaagatt tgagaaagca tataggggaa agctttggtc catgtttttt
     1381 cagtggagtg cctggttcat tttatactag acttttccct tccaaaagtt tacattttgt
     1441 ttactcctcc tacagtctca tgtggctatc tcaggtgcct aatgggattg aaaataacaa
     1501 gggaaacatt tacatggcaa gaacaagccc tctaagtgtt attaaagcat actacaagca
     1561 atatgaaata gatttttcaa attttctcaa gtaccgttca gaggaattga tgaaaggtgg
     1621 aaagatggtg ttaacactcc taggtagaga aagtgaggat cctactagca aagaatgctg
     1681 ttacatttgg gagcttctag ccatggccct caataagttg gttgaagagg gattgataaa
     1741 agaagagaaa gtagatgcat tcaatattcc tcaatacaca ccatcaccag cagaagtaaa
     1801 gtacatagtt gagaaggaag gatcattcac cattaatcgc ttggaaacat caagagttca
     1861 ttggaatgct tctaataatg agaagaatgg tggttacaat gtgtcaaggt gcatgagagc
     1921 tgtggctgag cctttgcttg tcagccactt tgacaaggaa ttgatggatt tagtgttcca
     1981 caagtacgaa gagattgttt ctgattgcat gtccaaagag aatactgagt ttataaatgt
     2041 catcatctcc ttgaccaaaa taaattaata ctagagccag gcatcaaata aaacgaaagg
     2101 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2161 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//