##gff-version 3
##sequence-region BBa_J45180 1 2205
BBa_J45180	.	CDS	995	2068	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_J45180	.	start_codon	995	997	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45180	.	CDS	995	2068	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45180	.	stop_codon	2066	2068	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45180	.	terminator	2165	2205	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45180	.	stem_loop	2172	2191	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45180	.	stop_codon	2198	2198	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45180	.	polyA_site	2192	2205	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45180	.	engineered_region	2165	2205	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45180	.	promoter	1	57	.	+	0	ID=BBa_J45993;Name=BBa_J45993
BBa_J45180	.	promoter	22	27	.	+	0	ID=annotation1883648;Name=-35;Parent=BBa_J45993
BBa_J45180	.	promoter	46	51	.	+	0	ID=annotation1883647;Name=-10;Parent=BBa_J45993
BBa_J45180	.	engineered_region	66	967	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401
BBa_J45180	.	CDS	84	768	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_J45180	.	tag	704	737	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J45180	.	engineered_tag	744	768	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J45180	.	CDS	87	703	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J45180	.	promoter	914	967	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_J45180	.	engineered_region	914	967	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45180	.	promoter	933	938	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45180	.	non_covalent_binding_site	914	932	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45180	.	promoter	956	961	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45180	.	non_covalent_binding_site	939	957	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45180	.	terminator	777	905	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_J45180	.	terminator	865	905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J45180	.	stem_loop	872	891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45180	.	stop_codon	898	898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45180	.	polyA_site	892	905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45180	.	engineered_region	865	905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45180	.	terminator	777	856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J45180	.	stem_loop	788	831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45180	.	engineered_region	777	856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45180	.	ribosome_entry_site	66	77	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_J45180	.	terminator	2077	2156	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45180	.	stem_loop	2088	2131	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45180	.	engineered_region	2077	2156	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45180	.	ribosome_entry_site	976	988	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45180	.	engineered_region	976	988	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45180	.	ribosome_entry_site	982	985	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45180	.	ribosome_entry_site	976	988	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_J45180
gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaata
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatgga
ggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagc
aagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaa
cactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttt
tattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttga
gaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttc
caaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaag
ggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaa
attttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgagga
tcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgata
aaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaagg
aaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggtta
caatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtg
ttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttga
ccaaaataaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata