Wintergree
type: ComponentDefinition
persistent identity: BBa J45181
display id: BBa_J45181
version: 1
was generated by: iGEM to SBOL conversion
description: Exponential phase dependent wintergreen odor generator
created: 2007-02-10T12:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Yes. It comes from many subparts.
owned by: james
owned by: myers
mutable description: This part converts salicylic acid into methyl salicylate (a mint-smelling compound) in the exponential phase of cell growth. The part consists of a stationary phase promoter (J45992), connected to an inverter (Q04401) as well as the methyl salicylate protein generator (J45119). This part replaces the defective part J45180, which used the truncated version of the stationary phase promoter (J45993). This part did not function as it was designed due to an imbalance between the strength of the stationary phase promoter and the strength of the promoter in the inverter (R0040).
discontinued: false
dominant: false
experience: experience/Issues
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We needed the full osmY promoter strength to be relatively comparable to the R0040 promoter strength.
star: false
creator: Stephen Payne
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J45119
component: BBa_J45994
sequence annotation: BBa_J45994
sequence annotation: BBa_J45119
sequence: https://synbiohub.org/public/igem/BBa_J45181_sequence/1

BBa_J45119
type: Component
persistent identity: component2260490
display id: component2260490
version: 1
top level: Wintergree
definition: BSMT1
access: public

BBa_J45994
type: Component
persistent identity: component2260476
display id: component2260476
version: 1
top level: Wintergree
definition: Exponentia
access: public

BBa_J45994
type: SequenceAnnotation
persistent identity: annotation2260476
display id: annotation2260476
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
component: BBa_J45994

https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
type: Range
persistent identity: range2260476
display id: range2260476
version: 1
top level: Wintergree
direction: direction/reverse
start: 1
end: 1109
orientation: inline

BBa_J45119
type: SequenceAnnotation
persistent identity: annotation2260490
display id: annotation2260490
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
component: BBa_J45119

https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
type: Range
persistent identity: range2260490
display id: range2260490
version: 1
top level: Wintergree
direction: direction/reverse
start: 1118
end: 2347
orientation: inline

Exponentia
type: ComponentDefinition
persistent identity: BBa J45994
display id: BBa_J45994
version: 1
was generated by: iGEM to SBOL conversion
description: Exponential phase transcriptional control device
created: 2008-01-29T12:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: It is a composite part of <partinfo>BBa_J45992</partinfo> and <partinfo>BBa_Q04401</partinfo>.
owned by: james
owned by: myers
mutable description: This composite regulator device produces a transcriptional signal in early growth phase but not in stationary phase.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _84_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J45992
component: BBa_Q04401
sequence annotation: BBa_J45992
sequence annotation: BBa_Q04401
sequence: https://synbiohub.org/public/igem/BBa_J45994_sequence/1

BBa_J45992
type: Component
persistent identity: component1960525
display id: component1960525
version: 1
top level: Exponentia
definition: p(osmY)
access: public

BBa_Q04401
type: Component
persistent identity: component1960526
display id: component1960526
version: 1
top level: Exponentia
definition: tetR inver
access: public

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1960525
display id: annotation1960525
version: 1
top level: Exponentia
location: https://synbiohub.org/public/igem/BBa_J45994/annotation1960525/range1960525/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45994/annotation1960525/range1960525/1
type: Range
persistent identity: range1960525
display id: range1960525
version: 1
top level: Exponentia
direction: direction/reverse
start: 1
end: 199
orientation: inline

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation1960526
display id: annotation1960526
version: 1
top level: Exponentia
location: https://synbiohub.org/public/igem/BBa_J45994/annotation1960526/range1960526/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_J45994/annotation1960526/range1960526/1
type: Range
persistent identity: range1960526
display id: range1960526
version: 1
top level: Exponentia
direction: direction/reverse
start: 208
end: 1109
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45119
display id: BBa_J45119
version: 1
was generated by: iGEM to SBOL conversion
description: Wintergreen odor enzyme (BMST1) generator
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Derived from the early part J45098 and sister to J45099.
owned by: james
owned by: myers
mutable description: This composite part include a ribosome binding site (B0032 different from J45099), the BSMT coding region, and a terminator. This part is attached to different promoters.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Used different RBS.
star: true
creator: Boyuan Zhu
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_J45004
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45119_sequence/1

BBa_B0012
type: Component
persistent identity: component1894761
display id: component1894761
version: 1
top level: BSMT1
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1894759
display id: component1894759
version: 1
top level: BSMT1
definition: BBa_B0010
access: public

BBa_J45004
type: Component
persistent identity: component1894758
display id: component1894758
version: 1
top level: BSMT1
definition: BSMT1
access: public

BBa_B0032
type: Component
persistent identity: component1894753
display id: component1894753
version: 1
top level: BSMT1
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894753
display id: annotation1894753
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
type: Range
persistent identity: range1894753
display id: range1894753
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894758
display id: annotation1894758
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
type: Range
persistent identity: range1894758
display id: range1894758
version: 1
top level: BSMT1
direction: direction/reverse
start: 20
end: 1093
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894759
display id: annotation1894759
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
type: Range
persistent identity: range1894759
display id: range1894759
version: 1
top level: BSMT1
direction: direction/forward
start: 1102
end: 1181
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894761
display id: annotation1894761
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
type: Range
persistent identity: range1894761
display id: range1894761
version: 1
top level: BSMT1
direction: direction/forward
start: 1190
end: 1230
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45992
display id: BBa_J45992
version: 1
was generated by: iGEM to SBOL conversion
description: Full-length stationary phase osmY promoter
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
star: false
creator: Stephen Payne
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J45992_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1883646
display id: annotation1883646
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
type: Range
persistent identity: range1883646
display id: range1883646
version: 1
top level: p(osmY)
direction: direction/forward
start: 164
end: 169
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1883645
display id: annotation1883645
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
type: Range
persistent identity: range1883645
display id: range1883645
version: 1
top level: p(osmY)
direction: direction/forward
start: 188
end: 193
orientation: inline

tetR inver
type: ComponentDefinition
persistent identity: BBa Q04401
display id: BBa_Q04401
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-derived transcriptional inverter
created: 2006-05-10T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0064
component: BBa_B0015
component: BBa_C0040
component: BBa_R0040
sequence annotation: BBa_B0064
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04401_sequence/1

BBa_B0064
type: Component
persistent identity: component2251459
display id: component2251459
version: 1
top level: tetR inver
definition: BBa_B0064
access: public

BBa_B0015
type: Component
persistent identity: component2251470
display id: component2251470
version: 1
top level: tetR inver
definition: BBa_B0015
access: public

BBa_C0040
type: Component
persistent identity: component2251463
display id: component2251463
version: 1
top level: tetR inver
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component2251471
display id: component2251471
version: 1
top level: tetR inver
definition: p(tetR)
access: public

BBa_B0064
type: SequenceAnnotation
persistent identity: annotation2251459
display id: annotation2251459
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
component: BBa_B0064

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
type: Range
persistent identity: range2251459
display id: range2251459
version: 1
top level: tetR inver
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251463
display id: annotation2251463
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
type: Range
persistent identity: range2251463
display id: range2251463
version: 1
top level: tetR inver
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251470
display id: annotation2251470
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
type: Range
persistent identity: range2251470
display id: range2251470
version: 1
top level: tetR inver
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251471
display id: annotation2251471
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
type: Range
persistent identity: range2251471
display id: range2251471
version: 1
top level: tetR inver
direction: direction/forward
start: 849
end: 902
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: iGEM to SBOL conversion
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Transport issues are being considered.
star: true
creator: Boyuan Zhu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BMST1
sequence annotation: start codon
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 1074
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
top level: BSMT1
direction: direction/forward
start: 1072
end: 1074
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: iGEM to SBOL conversion
description: Tuned RBS for Q04401
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Jason Kelly
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

https://synbiohub.org/public/igem/BBa_J45181_sequence/1
type: Sequence
persistent identity: BBa J45181 sequence
display id: BBa_J45181_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45994_sequence/1
type: Sequence
persistent identity: BBa J45994 sequence
display id: BBa_J45994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45119_sequence/1
type: Sequence
persistent identity: BBa J45119 sequence
display id: BBa_J45119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45992_sequence/1
type: Sequence
persistent identity: BBa J45992 sequence
display id: BBa_J45992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04401_sequence/1
type: Sequence
persistent identity: BBa Q04401 sequence
display id: BBa_Q04401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45004_sequence/1
type: Sequence
persistent identity: BBa J45004 sequence
display id: BBa_J45004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0064_sequence/1
type: Sequence
persistent identity: BBa B0064 sequence
display id: BBa_B0064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z