LOCUS       BBa_J45199              1739 bp    DNA     linear       18-Apr-2024
DEFINITION  Banana odor enzyme (ATF1) generator
ACCESSION   BBa_J45199
VERSION     BBa_J45199.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1602
                     /label=ATF1
     misc_feature    1169..1169
                     /label=t -> c
     misc_feature    1597..1602
                     /label=double TAA stop codon
     misc_feature    435..435
                     /label=a->g to eliminate EcoRI site
     CDS             22..1602
                     /label=BBa_J45014
     stem_loop       1622..1665
                     /label=stem_loop
     misc_feature    1611..1690
                     /label=BBa_B0010
     terminator      1611..1690
                     /label=BBa_B0010
     stem_loop       1706..1725
                     /label=T7 TE
     misc_feature    1732..1732
                     /label=stop
     polyA_site      1726..1739
                     /label=polya
     misc_feature    1699..1739
                     /label=BBa_B0012
     terminator      1699..1739
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaatgaa atcgatgaga aaaatcaggc ccccgtgcaa
       61 caagaatgcc tgaaagagat gattcagaat gggcatgctc ggcgtatggg atctgttgaa
      121 gatctgtatg ttgctctcaa cagacaaaac ttatatcgaa acttctgcac atatggagaa
      181 ttgagtgatt actgtactag ggatcagctc acattagctt tgagggaaat ctgcctgaaa
      241 aatccaactc ttttacatat tgttctacca acaagatggc caaatcatga aaattattat
      301 cgcagttccg aatactattc acggccacat ccagtgcatg attatatttc agtattacaa
      361 gaattgaaac tgagtggtgt ggttctcaat gaacaacctg agtacagtgc agtaatgaag
      421 caaatattag aagagttcaa aaatagtaag ggttcctata ctgcaaaaat ttttaaactt
      481 actaccactt tgactattcc ttactttgga ccaacaggac cgagttggcg gctaatttgt
      541 cttccagaag agcacacaga aaagtggaaa aaatttatct ttgtatctaa tcattgcatg
      601 tctgatggtc ggtcttcgat ccactttttt catgatttaa gagacgaatt aaataatatt
      661 aaaactccac caaaaaaatt agattacatt ttcaagtacg aggaggatta ccaattattg
      721 aggaaacttc cagaaccgat cgaaaaggtg atagacttta gaccaccgta cttgtttatt
      781 ccgaagtcac ttctttcggg tttcatctac aatcatttga gattttcttc aaaaggtgtc
      841 tgtatgagaa tggatgatgt ggaaaaaacc gatgatgttg tcaccgagat catcaatatt
      901 tcaccaacag aatttcaagc gattaaagca aatattaaat caaatatcca aggtaagtgt
      961 actatcactc cgtttttaca tgtttgttgg tttgtatctc ttcataaatg gggtaaattt
     1021 ttcaaaccat tgaacttcga atggcttacg gatattttta tccccgcaga ttgccgctca
     1081 caactaccag atgatgatga aatgagacag atgtacagat atggcgctaa cgttggattt
     1141 attgacttca ccccctggat aagcgaatct gacatgaatg ataacaaaga aaatttttgg
     1201 ccacttattg agcactacca tgaagtaatt tcggaagctt taagaaataa aaagcatctc
     1261 catggcttag ggttcaatat acaaggcttc gttcaaaaat atgtgaacat tgacaaggta
     1321 atgtgcgatc gtgccatcgg gaaaagacgc ggaggtacat tgttaagcaa tgtaggtctg
     1381 tttaatcagt tagaggagcc cgatgccaaa tattctatat gcgatttggc atttggccaa
     1441 tttcaaggat cctggcacca agcattttcc ttgggtgttt gttcgactaa tgtaaagggg
     1501 atgaatattg ttgttgcttc aacaaagaat gttgttggta gtcaagaatc tctcgaagag
     1561 ctttgctcca tttacaaagc tctcctttta ggcccttaat aatactagag ccaggcatca
     1621 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1681 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//