##gff-version 3
##sequence-region BBa_J45199 1 1739
BBa_J45199	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45199	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45199	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45199	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45199	.	terminator	1699	1739	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45199	.	polyA_site	1726	1739	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45199	.	engineered_region	1699	1739	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45199	.	stem_loop	1706	1725	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45199	.	stop_codon	1732	1732	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45199	.	CDS	22	1602	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45199	.	sequence_alteration	1169	1169	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45199	.	point_mutation	435	435	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45199	.	stop_codon	1597	1602	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45199	.	CDS	22	1602	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45199	.	terminator	1611	1690	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45199	.	stem_loop	1622	1665	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45199	.	engineered_region	1611	1690	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J45199
attaaagaggagaaatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagaga
tgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcg
aaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctg
aaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatact
attcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaaca
acctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaattttt
aaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagc
acacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttca
tgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattac
caattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcac
ttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaac
cgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatc
caaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaac
cattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgag
acagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataac
aaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatg
gcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaa
aagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgc
gatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaagggga
tgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagc
tctccttttaggcccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a