##gff-version 3
##sequence-region BBa_J45200 1 1801
BBa_J45200	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45200	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45200	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45200	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45200	.	terminator	1761	1801	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45200	.	polyA_site	1788	1801	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45200	.	engineered_region	1761	1801	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45200	.	stem_loop	1768	1787	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45200	.	stop_codon	1794	1794	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45200	.	terminator	1673	1752	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45200	.	stem_loop	1684	1727	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45200	.	engineered_region	1673	1752	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45200	.	CDS	84	1664	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45200	.	sequence_alteration	1231	1231	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45200	.	point_mutation	497	497	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45200	.	stop_codon	1659	1664	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45200	.	CDS	84	1664	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45200	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45200	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45200	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45200	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45200	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45200	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_J45200
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcat
gctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatg
gagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactctttt
acatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatcca
gtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcag
taatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccacttt
gactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaa
aaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaat
taaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaact
tccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatc
tacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccg
agatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactat
cactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatgg
cttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatg
gcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggcc
acttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatata
caaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacat
tgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggcca
atttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgct
tcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggccctt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata