LOCUS       BBa_J45219              1737 bp    DNA     linear       26-Dec-2024
DEFINITION  Banana odor enzyme (ATF1) generator
ACCESSION   BBa_J45219
VERSION     BBa_J45219.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..1600
                     /label=ATF1
     misc_feature    1167..1167
                     /label=t -> c
     misc_feature    1595..1600
                     /label=double TAA stop codon
     misc_feature    433..433
                     /label=a->g to eliminate EcoRI site
     CDS             20..1600
                     /label=BBa_J45014
     stem_loop       1620..1663
                     /label=stem_loop
     misc_feature    1609..1688
                     /label=BBa_B0010
     terminator      1609..1688
                     /label=BBa_B0010
     stem_loop       1704..1723
                     /label=T7 TE
     misc_feature    1730..1730
                     /label=stop
     polyA_site      1724..1737
                     /label=polya
     misc_feature    1697..1737
                     /label=BBa_B0012
     terminator      1697..1737
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgaatgaaat cgatgagaaa aatcaggccc ccgtgcaaca
       61 agaatgcctg aaagagatga ttcagaatgg gcatgctcgg cgtatgggat ctgttgaaga
      121 tctgtatgtt gctctcaaca gacaaaactt atatcgaaac ttctgcacat atggagaatt
      181 gagtgattac tgtactaggg atcagctcac attagctttg agggaaatct gcctgaaaaa
      241 tccaactctt ttacatattg ttctaccaac aagatggcca aatcatgaaa attattatcg
      301 cagttccgaa tactattcac ggccacatcc agtgcatgat tatatttcag tattacaaga
      361 attgaaactg agtggtgtgg ttctcaatga acaacctgag tacagtgcag taatgaagca
      421 aatattagaa gagttcaaaa atagtaaggg ttcctatact gcaaaaattt ttaaacttac
      481 taccactttg actattcctt actttggacc aacaggaccg agttggcggc taatttgtct
      541 tccagaagag cacacagaaa agtggaaaaa atttatcttt gtatctaatc attgcatgtc
      601 tgatggtcgg tcttcgatcc acttttttca tgatttaaga gacgaattaa ataatattaa
      661 aactccacca aaaaaattag attacatttt caagtacgag gaggattacc aattattgag
      721 gaaacttcca gaaccgatcg aaaaggtgat agactttaga ccaccgtact tgtttattcc
      781 gaagtcactt ctttcgggtt tcatctacaa tcatttgaga ttttcttcaa aaggtgtctg
      841 tatgagaatg gatgatgtgg aaaaaaccga tgatgttgtc accgagatca tcaatatttc
      901 accaacagaa tttcaagcga ttaaagcaaa tattaaatca aatatccaag gtaagtgtac
      961 tatcactccg tttttacatg tttgttggtt tgtatctctt cataaatggg gtaaattttt
     1021 caaaccattg aacttcgaat ggcttacgga tatttttatc cccgcagatt gccgctcaca
     1081 actaccagat gatgatgaaa tgagacagat gtacagatat ggcgctaacg ttggatttat
     1141 tgacttcacc ccctggataa gcgaatctga catgaatgat aacaaagaaa atttttggcc
     1201 acttattgag cactaccatg aagtaatttc ggaagcttta agaaataaaa agcatctcca
     1261 tggcttaggg ttcaatatac aaggcttcgt tcaaaaatat gtgaacattg acaaggtaat
     1321 gtgcgatcgt gccatcggga aaagacgcgg aggtacattg ttaagcaatg taggtctgtt
     1381 taatcagtta gaggagcccg atgccaaata ttctatatgc gatttggcat ttggccaatt
     1441 tcaaggatcc tggcaccaag cattttcctt gggtgtttgt tcgactaatg taaaggggat
     1501 gaatattgtt gttgcttcaa caaagaatgt tgttggtagt caagaatctc tcgaagagct
     1561 ttgctccatt tacaaagctc tccttttagg cccttaataa tactagagcc aggcatcaaa
     1621 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1681 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//