LOCUS       BBa_J45220              1800 bp    DNA     linear       22-Dec-2024
DEFINITION  Banana odor generator
ACCESSION   BBa_J45220
VERSION     BBa_J45220.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..1663
                     /label=ATF1
     misc_feature    1230..1230
                     /label=t -> c
     misc_feature    1658..1663
                     /label=double TAA stop codon
     misc_feature    496..496
                     /label=a->g to eliminate EcoRI site
     CDS             83..1663
                     /label=BBa_J45014
     stem_loop       1683..1726
                     /label=stem_loop
     misc_feature    1672..1751
                     /label=BBa_B0010
     terminator      1672..1751
                     /label=BBa_B0010
     stem_loop       1767..1786
                     /label=T7 TE
     misc_feature    1793..1793
                     /label=stop
     polyA_site      1787..1800
                     /label=polya
     misc_feature    1760..1800
                     /label=BBa_B0012
     terminator      1760..1800
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgaatga aatcgatgag aaaaatcagg cccccgtgca
      121 acaagaatgc ctgaaagaga tgattcagaa tgggcatgct cggcgtatgg gatctgttga
      181 agatctgtat gttgctctca acagacaaaa cttatatcga aacttctgca catatggaga
      241 attgagtgat tactgtacta gggatcagct cacattagct ttgagggaaa tctgcctgaa
      301 aaatccaact cttttacata ttgttctacc aacaagatgg ccaaatcatg aaaattatta
      361 tcgcagttcc gaatactatt cacggccaca tccagtgcat gattatattt cagtattaca
      421 agaattgaaa ctgagtggtg tggttctcaa tgaacaacct gagtacagtg cagtaatgaa
      481 gcaaatatta gaagagttca aaaatagtaa gggttcctat actgcaaaaa tttttaaact
      541 tactaccact ttgactattc cttactttgg accaacagga ccgagttggc ggctaatttg
      601 tcttccagaa gagcacacag aaaagtggaa aaaatttatc tttgtatcta atcattgcat
      661 gtctgatggt cggtcttcga tccacttttt tcatgattta agagacgaat taaataatat
      721 taaaactcca ccaaaaaaat tagattacat tttcaagtac gaggaggatt accaattatt
      781 gaggaaactt ccagaaccga tcgaaaaggt gatagacttt agaccaccgt acttgtttat
      841 tccgaagtca cttctttcgg gtttcatcta caatcatttg agattttctt caaaaggtgt
      901 ctgtatgaga atggatgatg tggaaaaaac cgatgatgtt gtcaccgaga tcatcaatat
      961 ttcaccaaca gaatttcaag cgattaaagc aaatattaaa tcaaatatcc aaggtaagtg
     1021 tactatcact ccgtttttac atgtttgttg gtttgtatct cttcataaat ggggtaaatt
     1081 tttcaaacca ttgaacttcg aatggcttac ggatattttt atccccgcag attgccgctc
     1141 acaactacca gatgatgatg aaatgagaca gatgtacaga tatggcgcta acgttggatt
     1201 tattgacttc accccctgga taagcgaatc tgacatgaat gataacaaag aaaatttttg
     1261 gccacttatt gagcactacc atgaagtaat ttcggaagct ttaagaaata aaaagcatct
     1321 ccatggctta gggttcaata tacaaggctt cgttcaaaaa tatgtgaaca ttgacaaggt
     1381 aatgtgcgat cgtgccatcg ggaaaagacg cggaggtaca ttgttaagca atgtaggtct
     1441 gtttaatcag ttagaggagc ccgatgccaa atattctata tgcgatttgg catttggcca
     1501 atttcaagga tcctggcacc aagcattttc cttgggtgtt tgttcgacta atgtaaaggg
     1561 gatgaatatt gttgttgctt caacaaagaa tgttgttggt agtcaagaat ctctcgaaga
     1621 gctttgctcc atttacaaag ctctcctttt aggcccttaa taatactaga gccaggcatc
     1681 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1741 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//