##gff-version 3
##sequence-region BBa_J45220 1 1800
BBa_J45220	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45220	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45220	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45220	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45220	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45220	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45220	.	terminator	1672	1751	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45220	.	engineered_region	1672	1751	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45220	.	stem_loop	1683	1726	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45220	.	terminator	1760	1800	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45220	.	engineered_region	1760	1800	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45220	.	polyA_site	1787	1800	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45220	.	stop_codon	1793	1793	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45220	.	stem_loop	1767	1786	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45220	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45220	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45220	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45220	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45220	.	CDS	83	1663	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45220	.	stop_codon	1658	1663	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45220	.	sequence_alteration	1230	1230	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45220	.	CDS	83	1663	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45220	.	point_mutation	496	496	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
>BBa_J45220
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatg
ctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatgg
agaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactctttta
catattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccag
tgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagt
aatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttg
actattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaa
aatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaatt
aaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaactt
ccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatct
acaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccga
gatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatc
actccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggc
ttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatgg
cgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggcca
cttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatac
aaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacatt
gttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaa
tttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgctt
caacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggccctta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata