LOCUS       BBa_J45250              1946 bp    DNA     linear       22-Dec-2024
DEFINITION  Stationary-phase-dependent banana odor generator
ACCESSION   BBa_J45250
VERSION     BBa_J45250.1  
FEATURES             Location/Qualifiers
     promoter        188..193
                     /label=-10
     promoter        164..169
                     /label=-35
     promoter        1..199
                     /label=BBa_J45992
     RBS             208..222
                     /label=RBS-1\Strong
     RBS             215..219
                     /label=RBS
     misc_feature    208..222
                     /label=BBa_B0030
     RBS             208..222
                     /label=BBa_B0030
     CDS             229..1809
                     /label=ATF1
     misc_feature    1376..1376
                     /label=t -> c
     misc_feature    1804..1809
                     /label=double TAA stop codon
     misc_feature    642..642
                     /label=a->g to eliminate EcoRI site
     CDS             229..1809
                     /label=BBa_J45014
     stem_loop       1829..1872
                     /label=stem_loop
     misc_feature    1818..1897
                     /label=BBa_B0010
     terminator      1818..1897
                     /label=BBa_B0010
     stem_loop       1913..1932
                     /label=T7 TE
     misc_feature    1939..1939
                     /label=stop
     polyA_site      1933..1946
                     /label=polya
     misc_feature    1906..1946
                     /label=BBa_B0012
     terminator      1906..1946
                     /label=BBa_B0012
ORIGIN
        1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg
       61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa
      121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
      181 tgtgatctat atttaacaat actagagatt aaagaggaga aatactagat gaatgaaatc
      241 gatgagaaaa atcaggcccc cgtgcaacaa gaatgcctga aagagatgat tcagaatggg
      301 catgctcggc gtatgggatc tgttgaagat ctgtatgttg ctctcaacag acaaaactta
      361 tatcgaaact tctgcacata tggagaattg agtgattact gtactaggga tcagctcaca
      421 ttagctttga gggaaatctg cctgaaaaat ccaactcttt tacatattgt tctaccaaca
      481 agatggccaa atcatgaaaa ttattatcgc agttccgaat actattcacg gccacatcca
      541 gtgcatgatt atatttcagt attacaagaa ttgaaactga gtggtgtggt tctcaatgaa
      601 caacctgagt acagtgcagt aatgaagcaa atattagaag agttcaaaaa tagtaagggt
      661 tcctatactg caaaaatttt taaacttact accactttga ctattcctta ctttggacca
      721 acaggaccga gttggcggct aatttgtctt ccagaagagc acacagaaaa gtggaaaaaa
      781 tttatctttg tatctaatca ttgcatgtct gatggtcggt cttcgatcca cttttttcat
      841 gatttaagag acgaattaaa taatattaaa actccaccaa aaaaattaga ttacattttc
      901 aagtacgagg aggattacca attattgagg aaacttccag aaccgatcga aaaggtgata
      961 gactttagac caccgtactt gtttattccg aagtcacttc tttcgggttt catctacaat
     1021 catttgagat tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga aaaaaccgat
     1081 gatgttgtca ccgagatcat caatatttca ccaacagaat ttcaagcgat taaagcaaat
     1141 attaaatcaa atatccaagg taagtgtact atcactccgt ttttacatgt ttgttggttt
     1201 gtatctcttc ataaatgggg taaatttttc aaaccattga acttcgaatg gcttacggat
     1261 atttttatcc ccgcagattg ccgctcacaa ctaccagatg atgatgaaat gagacagatg
     1321 tacagatatg gcgctaacgt tggatttatt gacttcaccc cctggataag cgaatctgac
     1381 atgaatgata acaaagaaaa tttttggcca cttattgagc actaccatga agtaatttcg
     1441 gaagctttaa gaaataaaaa gcatctccat ggcttagggt tcaatataca aggcttcgtt
     1501 caaaaatatg tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa aagacgcgga
     1561 ggtacattgt taagcaatgt aggtctgttt aatcagttag aggagcccga tgccaaatat
     1621 tctatatgcg atttggcatt tggccaattt caaggatcct ggcaccaagc attttccttg
     1681 ggtgtttgtt cgactaatgt aaaggggatg aatattgttg ttgcttcaac aaagaatgtt
     1741 gttggtagtc aagaatctct cgaagagctt tgctccattt acaaagctct ccttttaggc
     1801 ccttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1921 ttcgggtggg cctttctgcg tttata
//