LOCUS BBa_J45250 1946 bp DNA linear 22-Nov-2024 DEFINITION Stationary-phase-dependent banana odor generator ACCESSION BBa_J45250 VERSION BBa_J45250.1 FEATURES Location/Qualifiers promoter 188..193 /label=-10 promoter 164..169 /label=-35 promoter 1..199 /label=BBa_J45992 RBS 208..222 /label=RBS-1\Strong RBS 215..219 /label=RBS misc_feature 208..222 /label=BBa_B0030 RBS 208..222 /label=BBa_B0030 CDS 229..1809 /label=ATF1 misc_feature 1376..1376 /label=t -> c misc_feature 1804..1809 /label=double TAA stop codon misc_feature 642..642 /label=a->g to eliminate EcoRI site CDS 229..1809 /label=BBa_J45014 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 ORIGIN 1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg 61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa 121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat 181 tgtgatctat atttaacaat actagagatt aaagaggaga aatactagat gaatgaaatc 241 gatgagaaaa atcaggcccc cgtgcaacaa gaatgcctga aagagatgat tcagaatggg 301 catgctcggc gtatgggatc tgttgaagat ctgtatgttg ctctcaacag acaaaactta 361 tatcgaaact tctgcacata tggagaattg agtgattact gtactaggga tcagctcaca 421 ttagctttga gggaaatctg cctgaaaaat ccaactcttt tacatattgt tctaccaaca 481 agatggccaa atcatgaaaa ttattatcgc agttccgaat actattcacg gccacatcca 541 gtgcatgatt atatttcagt attacaagaa ttgaaactga gtggtgtggt tctcaatgaa 601 caacctgagt acagtgcagt aatgaagcaa atattagaag agttcaaaaa tagtaagggt 661 tcctatactg caaaaatttt taaacttact accactttga ctattcctta ctttggacca 721 acaggaccga gttggcggct aatttgtctt ccagaagagc acacagaaaa gtggaaaaaa 781 tttatctttg tatctaatca ttgcatgtct gatggtcggt cttcgatcca cttttttcat 841 gatttaagag acgaattaaa taatattaaa actccaccaa aaaaattaga ttacattttc 901 aagtacgagg aggattacca attattgagg aaacttccag aaccgatcga aaaggtgata 961 gactttagac caccgtactt gtttattccg aagtcacttc tttcgggttt catctacaat 1021 catttgagat tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga aaaaaccgat 1081 gatgttgtca ccgagatcat caatatttca ccaacagaat ttcaagcgat taaagcaaat 1141 attaaatcaa atatccaagg taagtgtact atcactccgt ttttacatgt ttgttggttt 1201 gtatctcttc ataaatgggg taaatttttc aaaccattga acttcgaatg gcttacggat 1261 atttttatcc ccgcagattg ccgctcacaa ctaccagatg atgatgaaat gagacagatg 1321 tacagatatg gcgctaacgt tggatttatt gacttcaccc cctggataag cgaatctgac 1381 atgaatgata acaaagaaaa tttttggcca cttattgagc actaccatga agtaatttcg 1441 gaagctttaa gaaataaaaa gcatctccat ggcttagggt tcaatataca aggcttcgtt 1501 caaaaatatg tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa aagacgcgga 1561 ggtacattgt taagcaatgt aggtctgttt aatcagttag aggagcccga tgccaaatat 1621 tctatatgcg atttggcatt tggccaattt caaggatcct ggcaccaagc attttccttg 1681 ggtgtttgtt cgactaatgt aaaggggatg aatattgttg ttgcttcaac aaagaatgtt 1741 gttggtagtc aagaatctct cgaagagctt tgctccattt acaaagctct ccttttaggc 1801 ccttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttata //