##gff-version 3
##sequence-region BBa_J45250 1 1946
BBa_J45250	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45250	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45250	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45250	.	promoter	1	199	.	+	0	ID=BBa_J45992;Name=BBa_J45992
BBa_J45250	.	promoter	188	193	.	+	0	ID=annotation1883645;Name=-10;Parent=BBa_J45992
BBa_J45250	.	promoter	164	169	.	+	0	ID=annotation1883646;Name=-35;Parent=BBa_J45992
BBa_J45250	.	CDS	229	1809	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45250	.	CDS	229	1809	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45250	.	point_mutation	642	642	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45250	.	sequence_alteration	1376	1376	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45250	.	stop_codon	1804	1809	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45250	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45250	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45250	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45250	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45250	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45250	.	ribosome_entry_site	208	222	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45250	.	engineered_region	208	222	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45250	.	ribosome_entry_site	215	219	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45250	.	ribosome_entry_site	208	222	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J45250
ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtg
gcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctg
atatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagatgaatgaa
atcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgg
gatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtga
ttactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttcta
ccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattata
tttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaat
attagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttac
tttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttg
tatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaa
aactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatc
gaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttga
gattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatat
ttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgttttta
catgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatattt
ttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttgg
atttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcac
taccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttc
aaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgt
aggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcc
tggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatg
ttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata