LOCUS       BBa_J45270              1802 bp    DNA     linear       25-Nov-2024
DEFINITION  Stationary-phase-dependent banana odor generator
ACCESSION   BBa_J45270
VERSION     BBa_J45270.1  
FEATURES             Location/Qualifiers
     promoter        46..51
                     /label=-10
     promoter        22..27
                     /label=-35
     promoter        1..57
                     /label=BBa_J45993
     RBS             66..78
                     /label=RBS-3\Weak
     RBS             72..75
                     /label=RBS
     misc_feature    66..78
                     /label=BBa_B0032
     RBS             66..78
                     /label=BBa_B0032
     CDS             85..1665
                     /label=ATF1
     misc_feature    1232..1232
                     /label=t -> c
     misc_feature    1660..1665
                     /label=double TAA stop codon
     misc_feature    498..498
                     /label=a->g to eliminate EcoRI site
     CDS             85..1665
                     /label=BBa_J45014
     stem_loop       1685..1728
                     /label=stem_loop
     misc_feature    1674..1753
                     /label=BBa_B0010
     terminator      1674..1753
                     /label=BBa_B0010
     stem_loop       1769..1788
                     /label=T7 TE
     misc_feature    1795..1795
                     /label=stop
     polyA_site      1789..1802
                     /label=polya
     misc_feature    1762..1802
                     /label=BBa_B0012
     terminator      1762..1802
                     /label=BBa_B0012
ORIGIN
        1 gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat ttaacaatac
       61 tagagtcaca caggaaagta ctagatgaat gaaatcgatg agaaaaatca ggcccccgtg
      121 caacaagaat gcctgaaaga gatgattcag aatgggcatg ctcggcgtat gggatctgtt
      181 gaagatctgt atgttgctct caacagacaa aacttatatc gaaacttctg cacatatgga
      241 gaattgagtg attactgtac tagggatcag ctcacattag ctttgaggga aatctgcctg
      301 aaaaatccaa ctcttttaca tattgttcta ccaacaagat ggccaaatca tgaaaattat
      361 tatcgcagtt ccgaatacta ttcacggcca catccagtgc atgattatat ttcagtatta
      421 caagaattga aactgagtgg tgtggttctc aatgaacaac ctgagtacag tgcagtaatg
      481 aagcaaatat tagaagagtt caaaaatagt aagggttcct atactgcaaa aatttttaaa
      541 cttactacca ctttgactat tccttacttt ggaccaacag gaccgagttg gcggctaatt
      601 tgtcttccag aagagcacac agaaaagtgg aaaaaattta tctttgtatc taatcattgc
      661 atgtctgatg gtcggtcttc gatccacttt tttcatgatt taagagacga attaaataat
      721 attaaaactc caccaaaaaa attagattac attttcaagt acgaggagga ttaccaatta
      781 ttgaggaaac ttccagaacc gatcgaaaag gtgatagact ttagaccacc gtacttgttt
      841 attccgaagt cacttctttc gggtttcatc tacaatcatt tgagattttc ttcaaaaggt
      901 gtctgtatga gaatggatga tgtggaaaaa accgatgatg ttgtcaccga gatcatcaat
      961 atttcaccaa cagaatttca agcgattaaa gcaaatatta aatcaaatat ccaaggtaag
     1021 tgtactatca ctccgttttt acatgtttgt tggtttgtat ctcttcataa atggggtaaa
     1081 tttttcaaac cattgaactt cgaatggctt acggatattt ttatccccgc agattgccgc
     1141 tcacaactac cagatgatga tgaaatgaga cagatgtaca gatatggcgc taacgttgga
     1201 tttattgact tcaccccctg gataagcgaa tctgacatga atgataacaa agaaaatttt
     1261 tggccactta ttgagcacta ccatgaagta atttcggaag ctttaagaaa taaaaagcat
     1321 ctccatggct tagggttcaa tatacaaggc ttcgttcaaa aatatgtgaa cattgacaag
     1381 gtaatgtgcg atcgtgccat cgggaaaaga cgcggaggta cattgttaag caatgtaggt
     1441 ctgtttaatc agttagagga gcccgatgcc aaatattcta tatgcgattt ggcatttggc
     1501 caatttcaag gatcctggca ccaagcattt tccttgggtg tttgttcgac taatgtaaag
     1561 gggatgaata ttgttgttgc ttcaacaaag aatgttgttg gtagtcaaga atctctcgaa
     1621 gagctttgct ccatttacaa agctctcctt ttaggccctt aataatacta gagccaggca
     1681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1741 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1801 ta
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