##gff-version 3
##sequence-region BBa_J45270 1 1802
BBa_J45270	.	promoter	1	57	.	+	0	ID=BBa_J45993;Name=BBa_J45993
BBa_J45270	.	promoter	22	27	.	+	0	ID=annotation1883648;Name=-35;Parent=BBa_J45993
BBa_J45270	.	promoter	46	51	.	+	0	ID=annotation1883647;Name=-10;Parent=BBa_J45993
BBa_J45270	.	terminator	1762	1802	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45270	.	stem_loop	1769	1788	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45270	.	stop_codon	1795	1795	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45270	.	polyA_site	1789	1802	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45270	.	engineered_region	1762	1802	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45270	.	ribosome_entry_site	66	78	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45270	.	engineered_region	66	78	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45270	.	ribosome_entry_site	72	75	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45270	.	ribosome_entry_site	66	78	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45270	.	terminator	1674	1753	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45270	.	stem_loop	1685	1728	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45270	.	engineered_region	1674	1753	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45270	.	CDS	85	1665	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45270	.	stop_codon	1660	1665	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45270	.	CDS	85	1665	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45270	.	point_mutation	498	498	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45270	.	sequence_alteration	1232	1232	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
>BBa_J45270
gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagtcacacaggaaagt
actagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggca
tgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatat
ggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttt
tacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatcc
agtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgca
gtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactt
tgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaa
aaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaa
ttaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaac
ttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcat
ctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcacc
gagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtacta
tcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatg
gcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatat
ggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggc
cacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatat
acaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtaca
ttgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggcc
aatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgc
ttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggccct
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata