LOCUS       BBa_J45298              1873 bp    DNA     linear       23-May-2024
DEFINITION  PchA & PchB enzyme generator
ACCESSION   BBa_J45298
VERSION     BBa_J45298.1  
FEATURES             Location/Qualifiers
     misc_feature    440..440
                     /label=g -> a to eliminate PstI site
     misc_feature    303..305
                     /label=pchA start codon
     misc_feature    1..3
                     /label=pchB start codon
     misc_feature    304..306
                     /label=pchB stop codon
     misc_feature    1731..1736
                     /label=pchA double TAA stop codon
     CDS             1..306
                     /label=PchB
     CDS             303..1736
                     /label=PchA
     CDS             1..1736
                     /label=BBa_J45017
     stem_loop       1756..1799
                     /label=stem_loop
     misc_feature    1745..1824
                     /label=BBa_B0010
     terminator      1745..1824
                     /label=BBa_B0010
     stem_loop       1840..1859
                     /label=T7 TE
     misc_feature    1866..1866
                     /label=stop
     polyA_site      1860..1873
                     /label=polya
     misc_feature    1833..1873
                     /label=BBa_B0012
     terminator      1833..1873
                     /label=BBa_B0012
ORIGIN
        1 atgaaaactc ccgaagactg caccggcctg gcggacatcc gcgaggccat cgaccggatc
       61 gacctggata tcgtccaggc cctcggccgc cgcatggact acgtcaaggc ggcgtcgcgc
      121 ttcaaggcca gcgaggcggc gattccggcg cccgagcggg tcgccgcgat gctccccgag
      181 cgcgcccgct gggccgagga aaacggactc gacgcgccct tcgtcgaggg actgttcgcg
      241 cagatcatcc actggtacat cgccgagcag atcaagtact ggcgccagac acggggtgcc
      301 gcatgagccg gctggcgccc ctgagccagt gcctgcacgc cttgcgcggc accttcgagc
      361 gcgccatcgg ccaggcgcag gcgctcgatc gtccggtgct ggtggcggca tcgttcgaga
      421 tcgacccatt ggacccgcta caggtattcg gtgcctggga cgaccggcaa acgccctgcc
      481 tgtactggga acagcccgag ctggcgttct tcgcctgggg ctgcgccctg gagctgcaag
      541 gccacggcga acagcgcttc gcccggatcg aggaaaactg gcaattgctc tgcgccgacg
      601 ccgtggtcga gggcccgctg gcgccgcgcc tgtgcggcgg attccgcttc gatccgcgcg
      661 gcccgcgcga ggaacactgg caagccttcg ccgatgccag cctgatgctc gccggcatca
      721 ccgtgctgcg cgagggcgaa cgctaccggg tactctgcca acacctggcc aagcccggcg
      781 aagatgccct ggccctggcc gcctaccact gctcggcgct actgcgcctg aggcagccgg
      841 ccagacgccg gccctcgggg ccgaccgctg gcgcgcaggg cgacgcttcg gcgcaggagc
      901 gcaggcaatg ggaagccaag gtgagcgacg cggtaagcag tgtccgccag ggacgcttcg
      961 gcaaggtcgt gctggcccgc acccaggccc ggcctctcgg cgacatcgag ccgtggcagg
     1021 tcatcgaaca cctgcgtctg caacatgccg acgcccagct gttcgcctgt cgccgcggca
     1081 acgcctgctt cctcggcgcc tccccggaac gcctggtccg cattcgcgcc ggcgaggcac
     1141 tcacccatgc cctggccggg accatcgccc gcggcggcga tgcccaggaa gatgcgcggc
     1201 tcggacaggc cctgctggac agcgccaagg acaggcacga acaccagttg gtggtggagg
     1261 cgatccgtac ggccctggaa cccttcagcg aggtgctgga aatccccgat gcgcccggcc
     1321 tgaaacgact ggcgcgagtc cagcacctga acacgccgat ccgcgcccgc ctcgctgacg
     1381 caggcggcat cctgcggctg ctacaagcgc tgcatccgac ccccgcggtg ggcggctacc
     1441 cacgcagcgc ggcgctggac tacatccgcc agcacgaagg gatggaccgc ggctggtacg
     1501 ccgcgccgct gggctggctc gacggcgaag gcaacggcga tttcctggtg gcgctgcgct
     1561 cggccctgct cacgccgggc cggggctacc tgttcgccgg ctgcggtctg gtaggcgatt
     1621 cggaaccggc ccacgagtat cgcgaaacct gccttaagct cagtgccatg cgggaagctc
     1681 tatccgccat aggcggcctg gacgaagtgc ccttgcagcg cggcgtcgcc taataatact
     1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1861 ttctgcgttt ata
//