##gff-version 3
##sequence-region BBa_J45298 1 1873
BBa_J45298	.	CDS	1	1736	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45298	.	stop_codon	1731	1736	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45298	.	start_codon	303	305	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45298	.	CDS	303	1736	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45298	.	start_codon	1	3	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45298	.	stop_codon	304	306	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45298	.	point_mutation	440	440	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45298	.	CDS	1	306	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45298	.	terminator	1833	1873	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45298	.	polyA_site	1860	1873	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45298	.	engineered_region	1833	1873	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45298	.	stem_loop	1840	1859	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45298	.	stop_codon	1866	1866	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45298	.	terminator	1745	1824	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45298	.	stem_loop	1756	1799	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45298	.	engineered_region	1745	1824	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J45298
atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccagg
ccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcg
ggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttc
gcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggc
gcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccg
gtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgc
cctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaaca
gcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctg
tgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcg
ccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccct
ggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgct
ggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgcc
agggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcga
acacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccg
gaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgccc
aggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgat
ccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccag
cacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgaccc
ccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgc
cgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggc
cggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagc
tcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata