LOCUS       BBa_J45299              1894 bp    DNA     linear       22-Nov-2024
DEFINITION  PchA & PchB enzyme generator
ACCESSION   BBa_J45299
VERSION     BBa_J45299.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    461..461
                     /label=g -> a to eliminate PstI site
     misc_feature    324..326
                     /label=pchA start codon
     misc_feature    22..24
                     /label=pchB start codon
     misc_feature    325..327
                     /label=pchB stop codon
     misc_feature    1752..1757
                     /label=pchA double TAA stop codon
     CDS             22..327
                     /label=PchB
     CDS             324..1757
                     /label=PchA
     CDS             22..1757
                     /label=BBa_J45017
     stem_loop       1777..1820
                     /label=stem_loop
     misc_feature    1766..1845
                     /label=BBa_B0010
     terminator      1766..1845
                     /label=BBa_B0010
     stem_loop       1861..1880
                     /label=T7 TE
     misc_feature    1887..1887
                     /label=stop
     polyA_site      1881..1894
                     /label=polya
     misc_feature    1854..1894
                     /label=BBa_B0012
     terminator      1854..1894
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaaaact cccgaagact gcaccggcct ggcggacatc
       61 cgcgaggcca tcgaccggat cgacctggat atcgtccagg ccctcggccg ccgcatggac
      121 tacgtcaagg cggcgtcgcg cttcaaggcc agcgaggcgg cgattccggc gcccgagcgg
      181 gtcgccgcga tgctccccga gcgcgcccgc tgggccgagg aaaacggact cgacgcgccc
      241 ttcgtcgagg gactgttcgc gcagatcatc cactggtaca tcgccgagca gatcaagtac
      301 tggcgccaga cacggggtgc cgcatgagcc ggctggcgcc cctgagccag tgcctgcacg
      361 ccttgcgcgg caccttcgag cgcgccatcg gccaggcgca ggcgctcgat cgtccggtgc
      421 tggtggcggc atcgttcgag atcgacccat tggacccgct acaggtattc ggtgcctggg
      481 acgaccggca aacgccctgc ctgtactggg aacagcccga gctggcgttc ttcgcctggg
      541 gctgcgccct ggagctgcaa ggccacggcg aacagcgctt cgcccggatc gaggaaaact
      601 ggcaattgct ctgcgccgac gccgtggtcg agggcccgct ggcgccgcgc ctgtgcggcg
      661 gattccgctt cgatccgcgc ggcccgcgcg aggaacactg gcaagccttc gccgatgcca
      721 gcctgatgct cgccggcatc accgtgctgc gcgagggcga acgctaccgg gtactctgcc
      781 aacacctggc caagcccggc gaagatgccc tggccctggc cgcctaccac tgctcggcgc
      841 tactgcgcct gaggcagccg gccagacgcc ggccctcggg gccgaccgct ggcgcgcagg
      901 gcgacgcttc ggcgcaggag cgcaggcaat gggaagccaa ggtgagcgac gcggtaagca
      961 gtgtccgcca gggacgcttc ggcaaggtcg tgctggcccg cacccaggcc cggcctctcg
     1021 gcgacatcga gccgtggcag gtcatcgaac acctgcgtct gcaacatgcc gacgcccagc
     1081 tgttcgcctg tcgccgcggc aacgcctgct tcctcggcgc ctccccggaa cgcctggtcc
     1141 gcattcgcgc cggcgaggca ctcacccatg ccctggccgg gaccatcgcc cgcggcggcg
     1201 atgcccagga agatgcgcgg ctcggacagg ccctgctgga cagcgccaag gacaggcacg
     1261 aacaccagtt ggtggtggag gcgatccgta cggccctgga acccttcagc gaggtgctgg
     1321 aaatccccga tgcgcccggc ctgaaacgac tggcgcgagt ccagcacctg aacacgccga
     1381 tccgcgcccg cctcgctgac gcaggcggca tcctgcggct gctacaagcg ctgcatccga
     1441 cccccgcggt gggcggctac ccacgcagcg cggcgctgga ctacatccgc cagcacgaag
     1501 ggatggaccg cggctggtac gccgcgccgc tgggctggct cgacggcgaa ggcaacggcg
     1561 atttcctggt ggcgctgcgc tcggccctgc tcacgccggg ccggggctac ctgttcgccg
     1621 gctgcggtct ggtaggcgat tcggaaccgg cccacgagta tcgcgaaacc tgccttaagc
     1681 tcagtgccat gcgggaagct ctatccgcca taggcggcct ggacgaagtg cccttgcagc
     1741 gcggcgtcgc ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1801 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1861 ggctcacctt cgggtgggcc tttctgcgtt tata
//